Generation of contractile actomyosin bundles depends on mechanosensitive actin filament assembly and disassembly

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Tojkander , S , Gateva , G , Husain , A , Krishnan , R & Lappalainen , P 2015 , ' Generation of contractile actomyosin bundles depends on mechanosensitive actin filament assembly and disassembly ' , eLife , vol. 4 , 06126 . https://doi.org/10.7554/eLife.06126

Title: Generation of contractile actomyosin bundles depends on mechanosensitive actin filament assembly and disassembly
Author: Tojkander, Sari; Gateva, Gergana; Husain, Amjad; Krishnan, Ramaswamy; Lappalainen, Pekka
Contributor: University of Helsinki, Institute of Biotechnology
University of Helsinki, Institute of Biotechnology
University of Helsinki, Institute of Biotechnology
Date: 2015-12-10
Language: eng
Number of pages: 28
Belongs to series: eLife
ISSN: 2050-084X
URI: http://hdl.handle.net/10138/166768
Abstract: Adhesion and morphogenesis of many non-muscle cells are guided by contractile actomyosin bundles called ventral stress fibers. While it is well established that stress fibers are mechanosensitive structures, physical mechanisms by which they assemble, align, and mature have remained elusive. Here we show that arcs, which serve as precursors for ventral stress fibers, undergo lateral fusion during their centripetal flow to form thick actomyosin bundles that apply tension to focal adhesions at their ends. Importantly, this myosin II-derived force inhibits vectorial actin polymerization at focal adhesions through AMPK-mediated phosphorylation of VASP, and thereby halts stress fiber elongation and ensures their proper contractility. Stress fiber maturation additionally requires ADF/cofilin-mediated disassembly of non-contractile stress fibers, whereas contractile fibers are protected from severing. Taken together, these data reveal that myosin-derived tension precisely controls both actin filament assembly and disassembly to ensure generation and proper alignment of contractile stress fibers in migrating cells.
Subject: VASODILATOR-STIMULATED PHOSPHOPROTEIN
PROTEIN-KINASE PHOSPHORYLATION
FOCAL ADHESION MATURATION
DORSAL STRESS FIBERS
F-ACTIN
DEPOLYMERIZING FACTOR
INTEGRIN ADHESOME
RETROGRADE FLOW
MIGRATING CELLS
HUMAN PLATELETS
1182 Biochemistry, cell and molecular biology
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