Application of aptamers in diagnostics, drug-delivery and imaging

Show full item record



Permalink

http://hdl.handle.net/10138/166773

Citation

Chandola , C , Kalme , S , Casteleijn , M G , Urtti , A & Neerathilingam , M 2016 , ' Application of aptamers in diagnostics, drug-delivery and imaging ' , Journal of Biosciences , vol. 41 , no. 3 , pp. 535-561 . https://doi.org/10.1007/s12038-016-9632-y

Title: Application of aptamers in diagnostics, drug-delivery and imaging
Author: Chandola, Chetan; Kalme, Sheetal; Casteleijn, Marco G.; Urtti, Arto; Neerathilingam, Muniasamy
Contributor: University of Helsinki, Faculty of Pharmacy
University of Helsinki, Faculty of Pharmacy
Date: 2016-09
Language: eng
Number of pages: 27
Belongs to series: Journal of Biosciences
ISSN: 0250-5991
URI: http://hdl.handle.net/10138/166773
Abstract: Aptamers are small, single-stranded oligonucleotides (DNA or RNA) that bind to their target with high specificity and affinity. Although aptamers are analogous to antibodies for a wide range of target recognition and variety of applications, they have significant advantages over antibodies. Since aptamers have recently emerged as a class of biomolecules with an application in a wide array of fields, we need to summarize the latest developments herein. In this review we will discuss about the latest developments in using aptamers in diagnostics, drug delivery and imaging. We begin with diagnostics, discussing the application of aptamers for the detection of infective agents itself, antigens/toxins (bacteria), biomarkers (cancer), or a combination. The ease of conjugation and labelling of aptamers makes them a potential tool for diagnostics. Also, due to the reduced off-target effects of aptamers, their use as a potential drug delivery tool is emerging rapidly. Hence, we discuss their use in targeted delivery in conjugation with siRNAs, nanoparticles, liposomes, drugs and antibodies. Finally, we discuss about the conjugation strategies applicable for RNA and DNA aptamers for imaging. Their stability and self-assembly after heating makes them superior over protein-based binding molecules in terms of labelling and conjugation strategies.
Subject: Aptamer
cancer
conjugation-methods
diagnostics
drug-delivery
imaging
CELL-ADHESION-MOLECULE
BREAST-CANCER CELLS
ENDOTHELIAL GROWTH-FACTOR
IN-VITRO SELECTION
LIVE AFRICAN TRYPANOSOMES
RESONANCE ENERGY-TRANSFER
AVIAN INFLUENZA-VIRUS
LABEL-FREE DETECTION
STABLE RNA APTAMERS
MRI CONTRAST AGENT
3111 Biomedicine
317 Pharmacy
Rights:


Files in this item

Total number of downloads: Loading...

Files Size Format View
0535_0561.pdf 553.0Kb PDF View/Open

This item appears in the following Collection(s)

Show full item record