Laminin-511 and laminin-521-based matrices for efficient hepatic specification of human pluripotent stem cells

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Kanninen , L K , Harjumäki , R , Peltoniemi , P , Bogacheva , M S , Salmi , T , Porola , P , Niklander , J , Smutny , T , Urtti , A , Yliperttula , M L & Lou , Y-R 2016 , ' Laminin-511 and laminin-521-based matrices for efficient hepatic specification of human pluripotent stem cells ' , Biomaterials , vol. 103 , pp. 86-100 . https://doi.org/10.1016/j.biomaterials.2016.06.054

Title: Laminin-511 and laminin-521-based matrices for efficient hepatic specification of human pluripotent stem cells
Author: Kanninen, Liisa K.; Harjumäki, Riina; Peltoniemi, Pasi; Bogacheva, Mariia S.; Salmi, Tuuli; Porola, Pauliina; Niklander, Johanna; Smutny, Tomas; Urtti, Arto; Yliperttula, Marjo L.; Lou, Yan-Ru
Contributor: University of Helsinki, Faculty of Pharmacy
University of Helsinki, Faculty of Pharmacy
University of Helsinki, Faculty of Pharmacy
University of Helsinki, Faculty of Pharmacy
University of Helsinki, Clinicum
University of Helsinki, Faculty of Pharmacy
University of Helsinki, Faculty of Pharmacy
University of Helsinki, Faculty of Pharmacy
University of Helsinki, Faculty of Pharmacy
University of Helsinki, Faculty of Pharmacy
Date: 2016-10
Language: eng
Number of pages: 15
Belongs to series: Biomaterials
ISSN: 0142-9612
URI: http://hdl.handle.net/10138/167555
Abstract: Human pluripotent stem cells (hPSCs) have gained a solid foothold in basic research and drug industry as they can be used in vitro to study human development and have potential to offer limitless supply of various somatic cell types needed in drug development. Although the hepatic differentiation of hPSCs has been extensively studied, only a little attention has been paid to the role of the extracellular matrix. In this study we used laminin-511, laminin-521, and fibronectin, found in human liver progenitor cells, as culture matrices for hPSC-derived definitive endoderm cells. We observed that laminin-511 and laminin-521 either alone or in combination support the hepatic specification and that fibronectin is not a vital matrix protein for the hPSC-derived definitive endoderm cells. The expression of the laminin-511/521-specific integrins increased during the definitive endoderm induction and hepatic specification. The hepatic cells differentiated on laminin matrices showed the upregulation of liver-specific markers both at mRNA and protein levels, secreted human albumin, stored glycogen, and exhibited cytochrome P450 enzyme activity and inducibility. Altogether, we found that laminin-511 and laminin-521 can be used as stage-specific matrices to guide the hepatic specification of hPSC-derived definitive endoderm cells. 2016 The Author(s). Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license
Subject: Extracellular matrix
Human embryonic stem cell
Human induced pluripotent stem cell
Hepatic differentiation
Laminin-511
Laminin-521
HEPATOCYTE-LIKE CELLS
HUMAN FETAL HEPATOCYTES
PREGNANE X RECEPTOR
LONG-TERM CULTURE
EXTRACELLULAR-MATRIX
HUMAN LIVER
SELF-RENEWAL
CYTOCHROME-P450 ENZYMES
DIRECT DIFFERENTIATION
DEFINITIVE ENDODERM
318 Medical biotechnology
317 Pharmacy
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