Nuclear actin modulates cell motility via transcriptional regulation of adhesive and cytoskeletal genes

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dc.contributor University of Helsinki, Institute of Biotechnology en
dc.contributor.author Sharili, Amir S.
dc.contributor.author Kenny, Fiona N.
dc.contributor.author Vartiainen, Maria K.
dc.contributor.author Connelly, John T.
dc.date.accessioned 2016-10-20T09:51:02Z
dc.date.available 2016-10-20T09:51:02Z
dc.date.issued 2016-09-21
dc.identifier.citation Sharili , A S , Kenny , F N , Vartiainen , M K & Connelly , J T 2016 , ' Nuclear actin modulates cell motility via transcriptional regulation of adhesive and cytoskeletal genes ' , Scientific Reports , vol. 6 , 33893 . https://doi.org/10.1038/srep33893 en
dc.identifier.issn 2045-2322
dc.identifier.other PURE: 70116044
dc.identifier.other PURE UUID: 357b5c8d-8d96-4c01-9331-142f6c2f27fa
dc.identifier.other WOS: 000383578400001
dc.identifier.other Scopus: 84988632757
dc.identifier.other ORCID: /0000-0002-2017-0475/work/30287100
dc.identifier.uri http://hdl.handle.net/10138/167941
dc.description.abstract The actin cytoskeleton is a classic biomechanical mediator of cell migration. While it is known that actin also shuttles in and out of the nucleus, its functions within this compartment remain poorly understood. In this study, we investigated how nuclear actin regulates keratinocyte gene expression and cell behavior. Gene expression profiling of normal HaCaT keratinocytes compared to HaCaTs over-expressing wild-type beta-actin or beta-actin tagged with a nuclear localization sequence (NLS-actin), identified multiple adhesive and cytoskeletal genes, such as MYL9, ITGB1, and VCL, which were significantly down-regulated in keratinocytes with high levels of nuclear actin. In addition, genes associated with transcriptional regulation and apoptosis were up-regulated in cells over expressing NLS-actin. Functionally, accumulation of actin in the nucleus altered cytoskeletal and focal adhesion organization and inhibited cell motility. Exclusion of endogenous actin from the nucleus by knocking down Importin 9 reversed this phenotype and enhanced cell migration. Based on these findings, we conclude that the level of actin in the nucleus is a transcriptional regulator for tuning keratinocyte migration. en
dc.format.extent 9
dc.language.iso eng
dc.relation.ispartof Scientific Reports
dc.rights en
dc.subject SERUM RESPONSE FACTOR en
dc.subject DYNAMICS en
dc.subject SRF en
dc.subject OOCYTES en
dc.subject ANTIBODIES en
dc.subject COMPLEXES en
dc.subject BINDING en
dc.subject 1182 Biochemistry, cell and molecular biology en
dc.title Nuclear actin modulates cell motility via transcriptional regulation of adhesive and cytoskeletal genes en
dc.type Article
dc.description.version Peer reviewed
dc.identifier.doi https://doi.org/10.1038/srep33893
dc.type.uri info:eu-repo/semantics/other
dc.type.uri info:eu-repo/semantics/publishedVersion
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