Lactobacillus rhamnosus GG Outcompetes Enterococcus faecium via Mucus-Binding Pili : Evidence for a Novel and Heterospecific Probiotic Mechanism

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Tytgat , H L P , Douillard , F P , Reunanen , J , Rasinkangas , P , Hendrickx , A P A , Laine , P K , Paulin , L , Satokari , R & de Vos , W M 2016 , ' Lactobacillus rhamnosus GG Outcompetes Enterococcus faecium via Mucus-Binding Pili : Evidence for a Novel and Heterospecific Probiotic Mechanism ' , Applied and Environmental Microbiology , vol. 82 , no. 19 , pp. 5756-5762 . https://doi.org/10.1128/AEM.01243-16

Title: Lactobacillus rhamnosus GG Outcompetes Enterococcus faecium via Mucus-Binding Pili : Evidence for a Novel and Heterospecific Probiotic Mechanism
Author: Tytgat, Hanne L. P.; Douillard, Francois P.; Reunanen, Justus; Rasinkangas, Pia; Hendrickx, Antoni P. A.; Laine, Pia K.; Paulin, Lars; Satokari, Reetta; de Vos, Willem M.
Contributor: University of Helsinki, Departments of Faculty of Veterinary Medicine
University of Helsinki, Departments of Faculty of Veterinary Medicine
University of Helsinki, Departments of Faculty of Veterinary Medicine
University of Helsinki, Institute of Biotechnology
University of Helsinki, Institute of Biotechnology
University of Helsinki, Research Programs Unit
University of Helsinki, Medicum
Date: 2016-10
Language: eng
Number of pages: 7
Belongs to series: Applied and Environmental Microbiology
ISSN: 0099-2240
URI: http://hdl.handle.net/10138/167987
Abstract: Vancomycin-resistant enterococci (VRE) have become a major nosocomial threat. Enterococcus faecium is of special concern, as it can easily acquire new antibiotic resistances and is an excellent colonizer of the human intestinal tract. Several clinical studies have explored the potential use of beneficial bacteria to weed out opportunistic pathogens. Specifically, the widely studied Lactobacillus rhamnosus strain GG has been applied successfully in the context of VRE infections. Here, we provide new insight into the molecular mechanism underlying the effects of this model probiotic on VRE decolonization. Both clinical VRE isolates and L. rhamnosus GG express pili on their cell walls, which are the key modulators of their highly efficient colonization of the intestinal mucosa. We found that one of the VRE pilus clusters shares considerable sequence similarity with the SpaCBA-SrtC1 pilus cluster of L. rhamnosus GG. Remarkable immunological and functional similarities were discovered between the mucus-binding pili of L. rhamnosus GG and those of the clinical E. faecium strain E1165, which was characterized at the genome level. Moreover, E. faecium strain E1165 bound efficiently to mucus, which may be prevented by the presence of the mucus-binding SpaC protein or antibodies against L. rhamnosus GG or SpaC. These results present experimental support for a novel probiotic mechanism, in which the mucus-binding pili of L. rhamnosus GG prevent the binding of a potential pathogen to the host. Hence, we provide a molecular basis for the further exploitation of L. rhamnosus GG and its pilins for prophylaxis and treatment of VRE infections.
Subject: VANCOMYCIN-RESISTANT-ENTEROCOCCI
COMPARATIVE GENOMIC ANALYSIS
GRAM-POSITIVE BACTERIA
ADHESION
COLONIZATION
EPIDEMIOLOGY
PATHOGENESIS
INFECTIONS
BIOGENESIS
HEALTH
318 Medical biotechnology
3111 Biomedicine
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