The room temperature crystal structure of a bacterial phytochrome determined by serial femtosecond crystallography

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Edlund , P , Takala , H , Claesson , E , Henry , L , Dods , R , Lehtivuori , H , Panman , M , Pande , K , White , T , Nakane , T , Berntsson , O , Gustavsson , E , Bath , P , Modi , V , Roy-Chowdhury , S , Zook , J , Berntsen , P , Pandey , S , Poudyal , I , Tenboer , J , Kupitz , C , Barty , A , Fromme , P , Koralek , J D , Tanaka , T , Spence , J , Liang , M , Hunter , M S , Boutet , S , Nango , E , Moffat , K , Groenhof , G , Ihalainen , J , Stojkovic , E A , Schmidt , M & Westenhoff , S 2016 , ' The room temperature crystal structure of a bacterial phytochrome determined by serial femtosecond crystallography ' Scientific Reports , vol. 6 , 35279 . DOI: 10.1038/srep35279

Title: The room temperature crystal structure of a bacterial phytochrome determined by serial femtosecond crystallography
Author: Edlund, Petra; Takala, Heikki; Claesson, Elin; Henry, Leocadie; Dods, Robert; Lehtivuori, Heli; Panman, Matthijs; Pande, Kanupriya; White, Thomas; Nakane, Takanori; Berntsson, Oskar; Gustavsson, Emil; Bath, Petra; Modi, Vaibhav; Roy-Chowdhury, Shatabdi; Zook, James; Berntsen, Peter; Pandey, Suraj; Poudyal, Ishwor; Tenboer, Jason; Kupitz, Christopher; Barty, Anton; Fromme, Petra; Koralek, Jake D.; Tanaka, Tomoyuki; Spence, John; Liang, Mengning; Hunter, Mark S.; Boutet, Sebastien; Nango, Eriko; Moffat, Keith; Groenhof, Gerrit; Ihalainen, Janne; Stojkovic, Emina A.; Schmidt, Marius; Westenhoff, Sebastian
Contributor: University of Helsinki, Medicum
Date: 2016-10-19
Language: eng
Number of pages: 9
Belongs to series: Scientific Reports
ISSN: 2045-2322
URI: http://hdl.handle.net/10138/169276
Abstract: Phytochromes are a family of photoreceptors that control light responses of plants, fungi and bacteria. A sequence of structural changes, which is not yet fully understood, leads to activation of an output domain. Time-resolved serial femtosecond crystallography (SFX) can potentially shine light on these conformational changes. Here we report the room temperature crystal structure of the chromophore-binding domains of the Deinococcus radiodurans phytochrome at 2.1 angstrom resolution. The structure was obtained by serial femtosecond X-ray crystallography from microcrystals at an X-ray free electron laser. We find overall good agreement compared to a crystal structure at 1.35 angstrom resolution derived from conventional crystallography at cryogenic temperatures, which we also report here. The thioether linkage between chromophore and protein is subject to positional ambiguity at the synchrotron, but is fully resolved with SFX. The study paves the way for time-resolved structural investigations of the phytochrome photocycle with time-resolved SFX.
Subject: CHROMOPHORE-BINDING DOMAIN
X-RAY-DIFFRACTION
GROUND-STATE
RED LIGHT
PROTEIN
BACTERIOPHYTOCHROME
PHOTOCONVERSION
REARRANGEMENTS
TRANSDUCTION
FLUORESCENCE
3111 Biomedicine
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