Increased Oxidative Stress in the Proximal Stomach of Patients with Barrett's Esophagus and Adenocarcinoma of the Esophagus and Esophagogastric Junction

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http://hdl.handle.net/10138/169572

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Kauppi , J , Räsänen , J , Sihvo , E , Nieminen , U , Arkkila , P , Ahotupa , M & Salo , J 2016 , ' Increased Oxidative Stress in the Proximal Stomach of Patients with Barrett's Esophagus and Adenocarcinoma of the Esophagus and Esophagogastric Junction ' , Translational oncology , vol. 9 , no. 4 , pp. 336-339 . https://doi.org/10.1016/j.tranon.2016.06.004

Titel: Increased Oxidative Stress in the Proximal Stomach of Patients with Barrett's Esophagus and Adenocarcinoma of the Esophagus and Esophagogastric Junction
Författare: Kauppi, Juha; Räsänen, Jari; Sihvo, Eero; Nieminen, Urpo; Arkkila, Perttu; Ahotupa, Markku; Salo, Jarmo
Upphovmannens organisation: III kirurgian klinikka
Clinicum
Department of Surgery
Gastroenterologian yksikkö
Department of Medicine
Datum: 2016-08
Språk: eng
Sidantal: 4
Tillhör serie: Translational oncology
ISSN: 1936-5233
DOI: https://doi.org/10.1016/j.tranon.2016.06.004
Permanenta länken (URI): http://hdl.handle.net/10138/169572
Abstrakt: OBJECTIVES: Oxidative stress (OS) is an essential element in the pathogenesis of Barrett's esophagus (BE) and its transformation to adenocarcinoma (EAC). The state of OS in the proximal stomach of patients with BE and EAC is unknown. Isoprostanes are a specific marker of OS not previously used to determine OS from BE/EAC tissue samples. PATIENTS AND METHODS: OS was measured in 42 patients with BE (n = 9), EAC (n = 9), or both (n = 24) and 15 control patients. A STAT-8-Isoprostane EIA Kit served to identify 8-Isoprostanes (8-IP), and a Glutathione Assay Kit was used to measure glutathione reduced form(GSH) and glutathione oxidized form. An OxiSelect Oxidative DNA Damage ELISA Kit (8-OHdG) served to measure 8-OH-deoxyguanosine. RESULTS: The 8-IP (P = .039) and 8-OHdG (P = .008) levels were higher, and the GSH level lower (P = .031), in the proximal stomach of the study group than in that of the controls. Helicobacter pylori infection was present in 8% of the study patients. CONCLUSIONS: In the proximal stomach of BE and EAC patients, OS was elevated and antioxidative capacity was reduced. This finding suggests that the gastroesophageal reflux causing BE also induces oxidative stress in the proximal stomach and may contribute to the development of cancer in the proximal stomach and gastric cardia.
Subject: HELICOBACTER-PYLORI INFECTION
GASTROESOPHAGEAL-REFLUX
FREE-RADICALS
GASTRIC CARDIA
DNA-ADDUCTS
CANCER
GLUTATHIONE
CARCINOGENESIS
INFLAMMATION
EXPRESSION
3122 Cancers
3126 Surgery, anesthesiology, intensive care, radiology
3121 General medicine, internal medicine and other clinical medicine
Referentgranskad: Ja
Licens: cc_by_nc_nd
Användningsbegränsning: openAccess
Parallelpublicerad version: publishedVersion


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