Near-infrared bioluminescent proteins for two-color multimodal imaging

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http://hdl.handle.net/10138/169883

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Rumyantsev , K A , Turoverov , K K & Verkhusha , V 2016 , ' Near-infrared bioluminescent proteins for two-color multimodal imaging ' , Scientific Reports , vol. 6 , 36588 . https://doi.org/10.1038/srep36588

Title: Near-infrared bioluminescent proteins for two-color multimodal imaging
Author: Rumyantsev, Konstantin A.; Turoverov, Konstantin K.; Verkhusha, Vladislav
Contributor: University of Helsinki, Medicum
Date: 2016-11-11
Language: eng
Number of pages: 10
Belongs to series: Scientific Reports
ISSN: 2045-2322
URI: http://hdl.handle.net/10138/169883
Abstract: Bioluminescence imaging became a widely used technique for noninvasive study of biological processes in small animals. Bioluminescent probes with emission in near-infrared (NIR) spectral region confer the advantage of having deep tissue penetration capacity. However, there are a very limited number of currently available luciferases that exhibit NIR bioluminescence. Here, we engineered two novel chimeric probes based on RLuc8 luciferase fused with iRFP670 and iRFP720 NIR fluorescent proteins. Due to an intramolecular bioluminescence resonance energy transfer (BRET) between RLuc8 and iRFPs, the chimeric luciferases exhibit NIR bioluminescence with maxima at 670 nm and 720 nm, respectively. The 50 nm spectral shift between emissions of the two iRFP chimeras enables combined multicolor bioluminescence imaging (BLI) and the respective multicolor fluorescence imaging (FLI) of the iRFPs. We show that for subcutaneously implanted cells, NIR bioluminescence provided a 10-fold increase in sensitivity compared to NIR FLI. In deep tissues, NIR BLI enabled detection of as low as 10(4) cells. Both BLI and FLI allowed monitoring of tumor growth and metastasis from early to late stages. Multimodal imaging, which combines concurrent BLI and FLI, provides continuous spatiotemporal analysis of metastatic cells in animals, including their localization and quantification.
Subject: FLUORESCENT PROTEINS
RENILLA LUCIFERASE
BREAST-CANCER
SINGLE-CELL
FAR-RED
VIVO
MODELS
PHYTOCHROME
METASTASIS
TISSUES
3111 Biomedicine
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