Ligand-Bound GeneSwitch Causes Developmental Aberrations in Drosophila that Are Alleviated by the Alternative Oxidase

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http://hdl.handle.net/10138/172824

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Andjelkovic , A , Kemppainen , K K & Jacobs , H T 2016 , ' Ligand-Bound GeneSwitch Causes Developmental Aberrations in Drosophila that Are Alleviated by the Alternative Oxidase ' , G3 - Genes genomes genetics , vol. 6 , no. 9 , pp. 2839-2846 . https://doi.org/10.1534/g3.116.030882

Title: Ligand-Bound GeneSwitch Causes Developmental Aberrations in Drosophila that Are Alleviated by the Alternative Oxidase
Author: Andjelkovic, Ana; Kemppainen, Kia K.; Jacobs, Howard T.
Contributor: University of Helsinki, Institute of Biotechnology
Date: 2016-09
Language: eng
Number of pages: 8
Belongs to series: G3 - Genes genomes genetics
ISSN: 2160-1836
URI: http://hdl.handle.net/10138/172824
Abstract: Culture of Drosophila expressing the steroid-dependent GeneSwitch transcriptional activator under the control of the ubiquitous -tubulin promoter was found to produce extensive pupal lethality, as well as a range of dysmorphic adult phenotypes, in the presence of high concentrations of the inducing drug RU486. Prominent among these was cleft thorax, seen previously in flies bearing mutant alleles of the nuclear receptor Ultraspiracle and many other mutants, as well as notched wings, leg malformations, and bristle abnormalities. Neither the -tubulin-GeneSwitch driver nor the inducing drug on their own produced any of these effects. A second GeneSwitch driver, under the control of the daughterless promoter, which gave much lower and more tissue-restricted transgene expression, exhibited only mild bristle abnormalities in the presence of high levels of RU486. Coexpression of the alternative oxidase (AOX) from Ciona intestinalis produced a substantial shift in the developmental outcome toward a wild-type phenotype, which was dependent on the AOX expression level. Neither an enzymatically inactivated variant of AOX, nor GFP, or the alternative NADH dehydrogenase Ndi1 from yeast gave any such rescue. Users of the GeneSwitch system should be aware of the potential confounding effects of its application in developmental studies.
Subject: inducible transgenes
nuclear receptor
Drosophila
cleft thorax
notched wings
LIFE-SPAN
FEMALE DROSOPHILA
NUCLEAR RECEPTORS
GENE-EXPRESSION
THORAX CLOSURE
JNK PATHWAY
MELANOGASTER
PROTEIN
METAMORPHOSIS
DEFICIENCY
1184 Genetics, developmental biology, physiology
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