RNA sequencing of chorionic villi from recurrent pregnancy loss patients reveals impaired function of basic nuclear and cellular machinery

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Sober , S , Rull , K , Reiman , M , Ilisson , P , Mattila , P & Laan , M 2016 , ' RNA sequencing of chorionic villi from recurrent pregnancy loss patients reveals impaired function of basic nuclear and cellular machinery ' , Scientific Reports , vol. 6 , 38439 . https://doi.org/10.1038/srep38439

Title: RNA sequencing of chorionic villi from recurrent pregnancy loss patients reveals impaired function of basic nuclear and cellular machinery
Author: Sober, Siim; Rull, Kristiina; Reiman, Mario; Ilisson, Piret; Mattila, Pirkko; Laan, Maris
Contributor: University of Helsinki, Institute for Molecular Medicine Finland
Date: 2016-12-08
Language: eng
Number of pages: 14
Belongs to series: Scientific Reports
ISSN: 2045-2322
URI: http://hdl.handle.net/10138/172962
Abstract: Recurrent pregnancy loss (RPL) concerns similar to 3% of couples aiming at childbirth. In the current study, transcriptomes and miRNomes of 1st trimester placental chorionic villi were analysed for 2 RPL cases (>= 6 miscarriages) and normal, but electively terminated pregnancies (ETP; n = 8). Sequencing was performed on Illumina HiSeq 2000 platform. Differential expression analyses detected 51 (27%) transcripts with increased and 138 (73%) with decreased expression in RPL compared to ETP (DESeq: FDR P <0.1 and DESeq2: <0.05). RPL samples had substantially decreased transcript levels of histones, regulatory RNAs and genes involved in telomere, spliceosome, ribosomal, mitochondrial and intra-cellular signalling functions. Downregulated expression of HIST1H1B and HIST1H4A (Wilcoxon test, fc
Subject: PREMATURE OVARIAN FAILURE
FETAL-GROWTH RESTRICTION
GENE-EXPRESSION
MITOCHONDRIAL-FUNCTION
SPONTANEOUS-ABORTION
HUMAN PLACENTA
MISCARRIAGE
E2F
COMPLEMENT
TRANSCRIPTOMICS
3123 Gynaecology and paediatrics
3111 Biomedicine
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