Expansion induced microRNA changes in bone marrow mesenchymal stromal cells reveals interplay between immune regulation and cell cycle

Show full item record



Permalink

http://hdl.handle.net/10138/173646

Citation

Kilpinen , L , Parmar , A , Greco , D , Korhonen , M , Lehenkari , P , Saavalainen , P & Laitinen , S 2016 , ' Expansion induced microRNA changes in bone marrow mesenchymal stromal cells reveals interplay between immune regulation and cell cycle ' , Aging Clinical and Experimental Research , vol. 8 , no. 11 , pp. 2799-2813 . https://doi.org/10.18632/aging.101088

Title: Expansion induced microRNA changes in bone marrow mesenchymal stromal cells reveals interplay between immune regulation and cell cycle
Author: Kilpinen, Lotta; Parmar, Amarjit; Greco, Dario; Korhonen, Matti; Lehenkari, Petri; Saavalainen, Paivi; Laitinen, Saara
Contributor: University of Helsinki, Research Programs Unit
University of Helsinki, Institute of Biotechnology
University of Helsinki, Research Programs Unit
Date: 2016-11
Language: eng
Number of pages: 15
Belongs to series: Aging Clinical and Experimental Research
ISSN: 1594-0667
URI: http://hdl.handle.net/10138/173646
Abstract: Mesenchymal stromal cells (MSC) are currently used in many cell based therapies. Prior to use in therapy, extensive expansion is required. We used microarray profiling to investigate expansion induced miRNA and mRNA expression changes of bone marrow MSCs (BM-MSCs) derived from old and young donors. The expression levels of 36 miRNAs were altered in cells derived from the old and respectively 39 miRNAs were altered in cells derived from young donors. Of these, only 12 were differentially expressed in both young and old donor BM-MSCs, and their predicted target mRNAs, were mainly linked to cell proliferation and senescence. Further qPCR verification showed that the expression of miR-1915-3p, miR-1207, miR-3665, and miR-762 correlated with the expansion time at passage 8. Previously described BM-MSC-specific miRNA fingerprints were also detected but these remained unchanged during expansion. Interestingly, members of well-studied miR-17/92 cluster, involved in cell cycle regulation, aging and also development of immune system, were down regulated specifically in cells from old donors. The role of this cluster in MSC functionality is worth future studies since it links expansion, aging and immune system together.
Subject: senescence
microRNA
mesenchymal stromal cells
SOCS3
STEM-CELLS
T-CELLS
SENESCENCE
DIFFERENTIATION
IDENTIFICATION
EXPRESSION
HMGB2
MIRNA
PROLIFERATION
INFLAMMATION
1182 Biochemistry, cell and molecular biology
3111 Biomedicine
Rights:


Files in this item

Total number of downloads: Loading...

Files Size Format View
Expansion_induced_microRNA.pdf 1.014Mb PDF View/Open

This item appears in the following Collection(s)

Show full item record