Early epithelial signaling center governs tooth budding morphogenesis

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http://hdl.handle.net/10138/174149

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Ahtiainen , L , Uski , I , Thesleff , I & Mikkola , M L 2016 , ' Early epithelial signaling center governs tooth budding morphogenesis ' , Journal of Cell Biology , vol. 214 , no. 6 , pp. 753-767 . https://doi.org/10.1083/jcb.201512074

Title: Early epithelial signaling center governs tooth budding morphogenesis
Author: Ahtiainen, Laura; Uski, Isa; Thesleff, Irma; Mikkola, Marja L.
Contributor: University of Helsinki, Institute of Biotechnology
University of Helsinki, Institute of Biotechnology
University of Helsinki, Institute of Biotechnology
Date: 2016-09-12
Language: eng
Number of pages: 15
Belongs to series: Journal of Cell Biology
ISSN: 0021-9525
URI: http://hdl.handle.net/10138/174149
Abstract: During organogenesis, cell fate specification and patterning are regulated by signaling centers, specialized clusters of morphogen-expressing cells. In many organs, initiation of development is marked by bud formation, but the cellular mechanisms involved are ill defined. Here, we use the mouse incisor tooth as a model to study budding morphogenesis. We show that a group of nonproliferative epithelial cells emerges in the early tooth primordium and identify these cells as a signaling center. Confocal live imaging of tissue explants revealed that although these cells reorganize dynamically, they do not reenter the cell cycle or contribute to the growing tooth bud. Instead, budding is driven by proliferation of the neighboring cells. We demonstrate that the activity of the ectodysplasin/Edar/nuclear factor kappa B pathway is restricted to the signaling center, and its inactivation leads to fewer quiescent cells and a smaller bud. These data functionally link the signaling center size to organ size and imply that the early signaling center is a prerequisite for budding morphogenesis.
Subject: ECTODERMAL APPENDAGE MORPHOGENESIS
HAIR FOLLICLE DEVELOPMENT
NF-KAPPA-B
MAMMALIAN TOOTH
ENAMEL KNOT
INCISOR DEVELOPMENT
DENTAL MESENCHYME
SHH EXPRESSION
TABBY MOUSE
ECTODYSPLASIN
1182 Biochemistry, cell and molecular biology
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