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Comparative study of ranavirus isolates from cod (Gadus morhua) and turbot (Psetta maxima) with reference to other ranaviruses

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dc.contributor.author Ariel, Ellen
dc.contributor.author Holopainen, Riikka
dc.contributor.author Olesen, Niels Jörgen
dc.contributor.author Tapiovaara, Hannele
dc.date.accessioned 2010-08-17T08:44:19Z
dc.date.available 2010-08-17T08:44:19Z
dc.date.issued 2010-06
dc.identifier.uri http://hdl.handle.net/10138/17447
dc.description.abstract Two iridovirus isolates recovered from cod (Gadus morhua) and turbot (Psetta maxima) in Denmark were examined in parallel with a panel of other ranaviruses including frog virus 3 (FV3), the reference strain for the genus Ranavirus. The isolates were assessed according to their reactivity in immunofluoresent antibody tests (IFAT) using both homologous and heterologous antisera and their amplification in PCR using primers targeting five genomic regions. The corresponding PCR fragments were sequenced, and the sequences obtained were used in phylogenetic analysis. In addition, the pathogenicity to rainbow trout under experimental challenge conditions was investigated. The viruses were serologically and genetically closely related to highly pathogenic ranaviruses such as European catfish iridovirus (ECV), European sheatfish iridovirus (ESV) and epizootic haematopoietic necrosis virus (EHNV). The challenge trials indicate that rainbow trout fry cultured at 15 C are not target species for the virus isolates in the present panel. We suggest that the two isolates belong in the genus Ranavirus and propose the name Ranavirus maxima (Rmax) for the turbot isolate. en
dc.language.iso fi en
dc.publisher Springer en
dc.title Comparative study of ranavirus isolates from cod (Gadus morhua) and turbot (Psetta maxima) with reference to other ranaviruses en
dc.type Artikkeli en

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