Adaptive protein divergence of BMP ligands takes place under developmental and evolutionary constraints

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http://hdl.handle.net/10138/174903

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Tauscher , P , Gui , J & Shimmi , O 2016 , ' Adaptive protein divergence of BMP ligands takes place under developmental and evolutionary constraints ' , Development , vol. 143 , no. 20 , pp. 3742-3750 . https://doi.org/10.1242/dev.130427

Titel: Adaptive protein divergence of BMP ligands takes place under developmental and evolutionary constraints
Författare: Tauscher, Petra; Gui, Jinghua; Shimmi, Osamu
Medarbetare: University of Helsinki, Institute of Biotechnology
University of Helsinki, Institute of Biotechnology
University of Helsinki, Institute of Biotechnology
Datum: 2016-10-18
Språk: eng
Sidantal: 9
Tillhör serie: Development
ISSN: 0950-1991
Permanenta länken (URI): http://hdl.handle.net/10138/174903
Abstrakt: The bone morphogenetic protein (BMP) signaling network, comprising evolutionary conserved BMP2/4/Decapentaplegic (Dpp) and Chordin/Short gastrulation (Sog), is widely utilized for dorsal-ventral (DV) patterning during animal development. A similar network is required for posterior crossvein (PCV) formation in the Drosophila pupal wing. Although both transcriptional and post-transcriptional regulation of co-factors in the network gives rise to tissue-specific and species-specific properties, their mechanisms are incompletely understood. In Drosophila, BMP5/6/7/8-type ligands, Screw (Scw) and Glass bottom boat (Gbb), form heterodimers with Dpp for DV patterning and PCV development, respectively. Sequence analysis indicates that the Scw ligand contains two N-glycosylation motifs: one being highly conserved between BMP2/4- and BMP5/6/7/8-type ligands, and the other being Scw ligand specific. Our data reveal that N-glycosylation of the Scw ligand boosts BMP signaling both in cell culture and in the embryo. In contrast, N-glycosylation modifications of Gbb or Scw ligands reduce the consistency of PCV development. These results suggest that tolerance for structural changes of BMP5/6/7/8-type ligands is dependent on developmental constraints. Furthermore, gain and loss of N-glycosylation motifs in conserved signaling molecules under evolutionary constraints appear to constitute flexible modules to adapt to developmental processes.
Subject: 1184 Genetics, developmental biology, physiology
Drosophila melanogaster
PROTEIN EVOLUTION
1182 Biochemistry, cell and molecular biology
Glycosylation
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