Critically ill patients demonstrate large interpersonal variation in intestinal microbiota dysregulation : a pilot study

Show simple item record Lankelma, Jacqueline M. van Vught, Lonneke A. Belzer, Clara Schultz, Marcus J. van der Poll, Tom de Vos, Willem M. Wiersinga, W. Joost 2017-02-09T09:43:01Z 2017-02-09T09:43:01Z 2017-01
dc.identifier.citation Lankelma , J M , van Vught , L A , Belzer , C , Schultz , M J , van der Poll , T , de Vos , W M & Wiersinga , W J 2017 , ' Critically ill patients demonstrate large interpersonal variation in intestinal microbiota dysregulation : a pilot study ' , Intensive Care Medicine , vol. 43 , no. 1 , pp. 59-68 .
dc.identifier.other PURE: 79670198
dc.identifier.other PURE UUID: 2ad911d6-7265-4d62-af44-6d7633aa9d22
dc.identifier.other WOS: 000391360400006
dc.identifier.other Scopus: 84994761112
dc.description.abstract The intestinal microbiota has emerged as a virtual organ with essential functions in human physiology. Antibiotic-induced disruption of the microbiota in critically ill patients may have a negative influence on key energy resources and immunity. We set out to characterize the fecal microbiota composition in critically ill patients both with and without sepsis and to explore the use of microbiota-derived markers for clinical outcome measurements in this setting. In this prospective observational cohort study we analyzed the fecal microbiota of 34 patients admitted to the intensive care unit. Fifteen healthy subjects served as controls. The fecal microbiota was phylogenetically characterized by 16S rRNA gene sequencing, and associations with clinical outcome parameters were evaluated. A marked shift in fecal bacterial composition was seen in all septic and non-septic critically ill patients compared with controls, with extreme interindividual differences. In 13 of the 34 patients, a single bacterial genus made up > 50% of the gut microbiota; in 4 patients this was even > 75%. A significant decrease in bacterial diversity was observed in half of the patients. No associations were found between microbiota diversity, Firmicutes/Bacteroidetes ratio, or Gram-positive/Gram-negative ratio and outcome measurements such as complications and survival. We observed highly heterogeneous patterns of intestinal microbiota in both septic and non-septic critically ill patients. Nevertheless, some general patterns were observed, including disappearance of bacterial genera with important functions in host metabolism. More detailed knowledge of the short- and long-term health consequences of these major shifts in intestinal bacterial communities is needed. en
dc.format.extent 10
dc.language.iso eng
dc.relation.ispartof Intensive Care Medicine
dc.rights cc_by_nc
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject Gut microbiota
dc.subject Critically ill
dc.subject Intensive care unit
dc.subject Antibiotics
dc.subject Sepsis
dc.subject SEVERE SEPSIS
dc.subject DIVERSITY
dc.subject DEFINITIONS
dc.subject MORTALITY
dc.subject 3111 Biomedicine
dc.title Critically ill patients demonstrate large interpersonal variation in intestinal microbiota dysregulation : a pilot study en
dc.type Article
dc.contributor.organization Medicum
dc.contributor.organization Willem Meindert Vos de / Principal Investigator
dc.contributor.organization Immunobiology Research Program
dc.contributor.organization Research Programs Unit
dc.contributor.organization Department of Bacteriology and Immunology
dc.contributor.organization de Vos & Salonen group
dc.description.reviewstatus Peer reviewed
dc.relation.issn 0342-4642
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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