Screening of a Haloferax volcanii Transposon Library Reveals Novel Motility and Adhesion Mutants

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dc.contributor University of Helsinki, Research Programs Unit en Legerme, Georgio Yang, Evan Esquivel, Rianne N. Kiljunen, Saija Savilahti, Harri Pohlschroder, Mechthild 2017-02-20T11:57:01Z 2017-02-20T11:57:01Z 2016
dc.identifier.citation Legerme , G , Yang , E , Esquivel , R N , Kiljunen , S , Savilahti , H & Pohlschroder , M 2016 , ' Screening of a Haloferax volcanii Transposon Library Reveals Novel Motility and Adhesion Mutants ' , Life , vol. 6 , no. 4 , pp. 41 . en
dc.identifier.issn 2075-1729
dc.identifier.other PURE: 77675968
dc.identifier.other PURE UUID: 567ee4da-f0fc-457c-8878-5abb7be7258d
dc.identifier.other Bibtex: urn:65ee83107cd6b3e2fdef2aee01a1f5a1
dc.identifier.other Scopus: 85000402107
dc.identifier.other ORCID: /0000-0003-0461-7270/work/30514096
dc.description.abstract Archaea, like bacteria, use type IV pili to facilitate surface adhesion. Moreover, archaeal flagella—structures required for motility—share a common ancestry with type IV pili. While the characterization of archaeal homologs of bacterial type IV pilus biosynthesis components has revealed important aspects of flagellum and pilus biosynthesis and the mechanisms regulating motility and adhesion in archaea, many questions remain. Therefore, we screened a Haloferax volcanii transposon insertion library for motility mutants using motility plates and adhesion mutants, using an adapted air–liquid interface assay. Here, we identify 20 genes, previously unknown to affect motility or adhesion. These genes include potential novel regulatory genes that will help to unravel the mechanisms underpinning these processes. Both screens also identified distinct insertions within the genomic region lying between two chemotaxis genes, suggesting that chemotaxis not only plays a role in archaeal motility, but also in adhesion. Studying these genes, as well as hypothetical genes hvo_2512 and hvo_2876—also critical for both motility and adhesion—will likely elucidate how these two systems interact. Furthermore, this study underscores the usefulness of the transposon library to screen other archaeal cellular processes for specific phenotypic defects. fi
dc.format.extent 14
dc.language.iso eng
dc.relation.ispartof Life
dc.rights en
dc.subject 1183 Plant biology, microbiology, virology en
dc.subject 1184 Genetics, developmental biology, physiology en
dc.title Screening of a Haloferax volcanii Transposon Library Reveals Novel Motility and Adhesion Mutants en
dc.type Article
dc.description.version Peer reviewed
dc.type.uri info:eu-repo/semantics/other
dc.type.uri info:eu-repo/semantics/publishedVersion

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