FHOD1 formin is upregulated in melanomas and modifies proliferation and tumor growth

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http://hdl.handle.net/10138/176712

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Peippo , M , Gardberg , M , Lamminen , T , Kaipio , K , Carpen , O & Heuser , V D 2017 , ' FHOD1 formin is upregulated in melanomas and modifies proliferation and tumor growth ' , Experimental Cell Research , vol. 350 , no. 1 , pp. 267-278 . https://doi.org/10.1016/j.yexcr.2016.12.004

Title: FHOD1 formin is upregulated in melanomas and modifies proliferation and tumor growth
Author: Peippo, Minna; Gardberg, Maria; Lamminen, Tarja; Kaipio, Katja; Carpen, Olli; Heuser, Vanina D.
Contributor: University of Helsinki, Medicum
Date: 2017-01-01
Language: eng
Number of pages: 12
Belongs to series: Experimental Cell Research
ISSN: 0014-4827
URI: http://hdl.handle.net/10138/176712
Abstract: The functional properties of actin-regulating formin proteins are diverse and in many cases cell-type specific. FHOD1, a formin expressed predominantly in cells of mesenchymal lineage, bundles actin filaments and participates in maintenance of cell shape, migration and cellular protrusions. FHOD1 participates in cancer associated epithelial to mesenchymal transition (EMT) in oral squamous cell carcinoma and breast cancer. The role of FHOD1 in melanomas has not been characterized. Here, we show that FHOD1 expression is typically strong in cutaneous melanomas and cultured melanoma cells while the expression is low or absent in benign nevi. By using shRNA to knockdown FHOD1 in melanoma cells, we discovered that FHOD1 depleted cells are larger, rounder and have smaller focal adhesions and inferior migratory capacity as compared to control cells. Importantly, we found FHOD1 depleted cells to have reduced colony-forming capacity and attenuated tumor growth in vivo, a finding best explained by the reduced proliferation rate caused by cell cycle arrest. Unexpectedly, FHOD1 depletion did not prevent invasive growth at the tumor margins. These results suggest that FHOD1 participates in key cellular processes that are dysregulated in malignancy, but may not be essential for melanoma cell invasion.
Subject: Formin
FHOD1
Cytoskeleton
Actin
Melanoma
Tumorigenesis
SRE
MKL-1
MRTF-A
MAL
TAN LINE FORMATION
NUCLEAR-MOVEMENT
CELL-MIGRATION
ACTIN
ADHESION
EXPRESSION
PROTEIN
CANCER
REORGANIZATION
TRANSCRIPTION
3111 Biomedicine
3122 Cancers
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