Aging and serum exomiR content in women-effects of estrogenic hormone replacement therapy

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http://hdl.handle.net/10138/177046

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Kangas , R , Tormakangas , T , Fey , V , Pursiheimo , J , Miinalainen , I , Alen , M , Kaprio , J , Sipila , S , Saamanen , A-M , Kovanen , V & Laakkonen , E K 2017 , ' Aging and serum exomiR content in women-effects of estrogenic hormone replacement therapy ' Scientific Reports , vol. 7 , 42702 . DOI: 10.1038/srep42702

Title: Aging and serum exomiR content in women-effects of estrogenic hormone replacement therapy
Author: Kangas, Reeta; Tormakangas, Timo; Fey, Vidal; Pursiheimo, Juha; Miinalainen, Ilkka; Alen, Markku; Kaprio, Jaakko; Sipila, Sarianna; Saamanen, Anna-Marja; Kovanen, Vuokko; Laakkonen, Eija K.
Contributor: University of Helsinki, Institute for Molecular Medicine Finland
Date: 2017-02-14
Language: eng
Number of pages: 14
Belongs to series: Scientific Reports
ISSN: 2045-2322
URI: http://hdl.handle.net/10138/177046
Abstract: Exosomes participate in intercellular messaging by transporting bioactive lipid-, protein-and RNA-molecules and -complexes. The contents of the exosomes reflect the physiological status of an individual making exosomes promising targets for biomarker analyses. In the present study we extracted exosome microRNAs (exomiRs) from serum samples of premenopausal women (n = 8) and monozygotic postmenopausal twins (n = 10 female pairs), discordant for the use of estrogenic hormone replacement therapy (HRT), in order to see whether the age or/and the use of HRT associates with exomiR content. A total of 241 exomiRs were detected by next generation sequencing, 10 showing age, 14 HRT and 10 age + HRT-related differences. When comparing the groups, differentially expressed miRs were predicted to affect cell proliferation processes showing inactivation with younger age and HRT usage. MiR-106-5p, -148a-3p, -27-3p, -126-5p, -28-3p and -30a-5p were significantly associated with serum 17 beta-estradiol. MiRs formed two hierarchical clusters being indicative of positive or negative health outcomes involving associations with body composition, serum 17 beta-estradiol, fat-, glucose-and inflammatory markers. Circulating exomiR clusters, obtained by NGS, could be used as indicators of metabolic and inflammatory status affected by hormonal changes at menopause. Furthermore, the individual effects of HRT-usage could be evaluated based on the serum exomiR signature.
Subject: MONOZYGOTIC TWIN PAIRS
POSTMENOPAUSAL WOMEN
BODY-COMPOSITION
SKELETAL-MUSCLE
BREAST-CANCER
MICRORNAS
EXPRESSION
DISCORDANT
CELLS
MENOPAUSE
3111 Biomedicine
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