Nagy , S , Polgar , P J , Andersson , M & Kovacs , A 2016 , ' Quick cytogenetic screening of breeding bulls using flow cytometric sperm DNA histogram analysis ' , Acta Veterinaria Hungarica , vol. 64 , no. 3 , pp. 372-379 . https://doi.org/10.1556/004.2016.035
Title: | Quick cytogenetic screening of breeding bulls using flow cytometric sperm DNA histogram analysis |
Author: | Nagy, Szabolcs; Polgar, Peter J.; Andersson, Magnus; Kovacs, Andras |
Contributor organization: | Departments of Faculty of Veterinary Medicine Production Animal Medicine |
Date: | 2016-09 |
Language: | eng |
Number of pages: | 8 |
Belongs to series: | Acta Veterinaria Hungarica |
ISSN: | 0236-6290 |
DOI: | https://doi.org/10.1556/004.2016.035 |
URI: | http://hdl.handle.net/10138/178238 |
Abstract: | The aim of the present study was to test the FXCycle PI/RNase kit for routine DNA analyses in order to detect breeding bulls and/or insemination doses carrying cytogenetic aberrations. In a series of experiments we first established basic DNA histogram parameters of cytogenetically healthy breeding bulls by measuring the intraspecific genome size variation of three animals, then we compared the histogram profiles of bulls carrying cytogenetic defects to the baseline values. With the exception of one case the test was able to identify bulls with cytogenetic defects. Therefore, we conclude that the assay could be incorporated into the laboratory routine where flow cytometry is applied for semen quality control. |
Subject: |
Cytogenetics
translocation sperm flow cytometry KOLMOGOROV-SMIRNOV TEST SPERMATOZOA RESOLUTION FERTILITY IDENTIFICATION TRANSLOCATION CHROMOSOMES PREJUDICE QUALITY BOARS 413 Veterinary science |
Peer reviewed: | Yes |
Rights: | cc_by_nc |
Usage restriction: | openAccess |
Self-archived version: | publishedVersion |
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