Quick cytogenetic screening of breeding bulls using flow cytometric sperm DNA histogram analysis

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Nagy , S , Polgar , P J , Andersson , M & Kovacs , A 2016 , ' Quick cytogenetic screening of breeding bulls using flow cytometric sperm DNA histogram analysis ' Acta Veterinaria Hungarica , vol. 64 , no. 3 , pp. 372-379 . DOI: 10.1556/004.2016.035

Title: Quick cytogenetic screening of breeding bulls using flow cytometric sperm DNA histogram analysis
Author: Nagy, Szabolcs; Polgar, Peter J.; Andersson, Magnus; Kovacs, Andras
Contributor: University of Helsinki, Production Animal Medicine
Date: 2016-09
Language: eng
Number of pages: 8
Belongs to series: Acta Veterinaria Hungarica
ISSN: 0236-6290
URI: http://hdl.handle.net/10138/178238
Abstract: The aim of the present study was to test the FXCycle PI/RNase kit for routine DNA analyses in order to detect breeding bulls and/or insemination doses carrying cytogenetic aberrations. In a series of experiments we first established basic DNA histogram parameters of cytogenetically healthy breeding bulls by measuring the intraspecific genome size variation of three animals, then we compared the histogram profiles of bulls carrying cytogenetic defects to the baseline values. With the exception of one case the test was able to identify bulls with cytogenetic defects. Therefore, we conclude that the assay could be incorporated into the laboratory routine where flow cytometry is applied for semen quality control.
Subject: Cytogenetics
translocation
sperm
flow cytometry
KOLMOGOROV-SMIRNOV TEST
SPERMATOZOA
RESOLUTION
FERTILITY
IDENTIFICATION
TRANSLOCATION
CHROMOSOMES
PREJUDICE
QUALITY
BOARS
413 Veterinary science
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