Pili-like proteins of Akkermansia muciniphila modulate host immune responses and gut barrier function

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Ottman , N , Reunanen , J , Meijerink , M , Pietilä , T , Kainulainen , V , Klievink , J , Huuskonen , L , Aalvink , S , Skurnik , M , Boeren , S , Satokari , R , Mercenier , A , Palva , A , Smidt , H , de Vos , W M & Belzer , C 2017 , ' Pili-like proteins of Akkermansia muciniphila modulate host immune responses and gut barrier function ' , PLoS One , vol. 12 , no. 3 , 0173004 . https://doi.org/10.1371/journal.pone.0173004

Title: Pili-like proteins of Akkermansia muciniphila modulate host immune responses and gut barrier function
Author: Ottman, Noora; Reunanen, Justus; Meijerink, Marjolein; Pietilä, Taija; Kainulainen, Veera; Klievink, Judith; Huuskonen, Laura; Aalvink, Steven; Skurnik, Mikael; Boeren, Sjef; Satokari, Reetta; Mercenier, Annick; Palva, Airi; Smidt, Hauke; de Vos, Willem M.; Belzer, Clara
Contributor organization: Biosciences
Veterinary Biosciences
Department of Pharmacology
Research Programs Unit
Immunobiology Research Program
Department of Bacteriology and Immunology
Hematologian yksikkö
Department of Medicine
Department of Oncology
Departments of Faculty of Veterinary Medicine
Mikael Skurnik / Principal Investigator
Reetta Maria Satokari / Principal Investigator
Airi Palva / Principal Investigator
Willem Meindert Vos de / Principal Investigator
de Vos & Salonen group
HUS Comprehensive Cancer Center
Date: 2017-03-01
Language: eng
Number of pages: 18
Belongs to series: PLoS One
ISSN: 1932-6203
DOI: https://doi.org/10.1371/journal.pone.0173004
URI: http://hdl.handle.net/10138/178655
Abstract: Gut barrier function is key in maintaining a balanced response between the host and its microbiome. The microbiota can modulate changes in gut barrier as well as metabolic and inflammatory responses. This highly complex system involves numerous microbiota-derived factors. The gut symbiont Akkermansia muciniphila is positively correlated with a lean phenotype, reduced body weight gain, amelioration of metabolic responses and restoration of gut barrier function by modulation of mucus layer thickness. However, the molecular mechanisms behind its metabolic and immunological regulatory properties are unexplored. Herein, we identify a highly abundant outer membrane pili-like protein of A. muciniphila MucT that is directly involved in immune regulation and enhancement of trans-epithelial resistance. The purified Amuc_1100 protein and enrichments containing all its associated proteins induced production of specific cytokines through activation of Toll-like receptor (TLR) 2 and TLR4. This mainly leads to high levels of IL-10 similar to those induced by the other beneficial immune suppressive microorganisms such as Faecalibacterium prausnitzii A2-165 and Lactobacillus plantarum WCFS1. Together these results indicate that outer membrane protein composition and particularly the newly identified highly abundant pili-like protein Amuc_1100 of A. muciniphila are involved in host immunological homeostasis at the gut mucosa, and improvement of gut barrier function.
3111 Biomedicine
Peer reviewed: Yes
Usage restriction: openAccess
Self-archived version: publishedVersion

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