Pili-like proteins of Akkermansia muciniphila modulate host immune responses and gut barrier function

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dc.contributor.author Ottman, Noora
dc.contributor.author Reunanen, Justus
dc.contributor.author Meijerink, Marjolein
dc.contributor.author Pietilä, Taija
dc.contributor.author Kainulainen, Veera
dc.contributor.author Klievink, Judith
dc.contributor.author Huuskonen, Laura
dc.contributor.author Aalvink, Steven
dc.contributor.author Skurnik, Mikael
dc.contributor.author Boeren, Sjef
dc.contributor.author Satokari, Reetta
dc.contributor.author Mercenier, Annick
dc.contributor.author Palva, Airi
dc.contributor.author Smidt, Hauke
dc.contributor.author de Vos, Willem M.
dc.contributor.author Belzer, Clara
dc.date.accessioned 2017-03-28T09:16:02Z
dc.date.available 2017-03-28T09:16:02Z
dc.date.issued 2017-03-01
dc.identifier.citation Ottman , N , Reunanen , J , Meijerink , M , Pietilä , T , Kainulainen , V , Klievink , J , Huuskonen , L , Aalvink , S , Skurnik , M , Boeren , S , Satokari , R , Mercenier , A , Palva , A , Smidt , H , de Vos , W M & Belzer , C 2017 , ' Pili-like proteins of Akkermansia muciniphila modulate host immune responses and gut barrier function ' , PLoS One , vol. 12 , no. 3 , 0173004 . https://doi.org/10.1371/journal.pone.0173004
dc.identifier.other PURE: 82123656
dc.identifier.other PURE UUID: b25b3729-e338-4176-8609-9af4df3eeeb9
dc.identifier.other WOS: 000395983500069
dc.identifier.other Scopus: 85014435525
dc.identifier.other ORCID: /0000-0001-8791-9260/work/37311564
dc.identifier.other ORCID: /0000-0001-5011-9294/work/31552395
dc.identifier.uri http://hdl.handle.net/10138/178655
dc.description.abstract Gut barrier function is key in maintaining a balanced response between the host and its microbiome. The microbiota can modulate changes in gut barrier as well as metabolic and inflammatory responses. This highly complex system involves numerous microbiota-derived factors. The gut symbiont Akkermansia muciniphila is positively correlated with a lean phenotype, reduced body weight gain, amelioration of metabolic responses and restoration of gut barrier function by modulation of mucus layer thickness. However, the molecular mechanisms behind its metabolic and immunological regulatory properties are unexplored. Herein, we identify a highly abundant outer membrane pili-like protein of A. muciniphila MucT that is directly involved in immune regulation and enhancement of trans-epithelial resistance. The purified Amuc_1100 protein and enrichments containing all its associated proteins induced production of specific cytokines through activation of Toll-like receptor (TLR) 2 and TLR4. This mainly leads to high levels of IL-10 similar to those induced by the other beneficial immune suppressive microorganisms such as Faecalibacterium prausnitzii A2-165 and Lactobacillus plantarum WCFS1. Together these results indicate that outer membrane protein composition and particularly the newly identified highly abundant pili-like protein Amuc_1100 of A. muciniphila are involved in host immunological homeostasis at the gut mucosa, and improvement of gut barrier function. en
dc.format.extent 18
dc.language.iso eng
dc.relation.ispartof PLoS One
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject INTESTINAL EPITHELIUM
dc.subject BACTEROIDES-FRAGILIS
dc.subject ULCERATIVE-COLITIS
dc.subject GEN. NOV.
dc.subject IN-VITRO
dc.subject MICROBIOTA
dc.subject BACTERIA
dc.subject MUCIN
dc.subject MICE
dc.subject ENTEROCYTES
dc.subject 3111 Biomedicine
dc.title Pili-like proteins of Akkermansia muciniphila modulate host immune responses and gut barrier function en
dc.type Article
dc.contributor.organization Biosciences
dc.contributor.organization Veterinary Biosciences
dc.contributor.organization Medicum
dc.contributor.organization Department of Pharmacology
dc.contributor.organization Research Programs Unit
dc.contributor.organization Immunobiology Research Program
dc.contributor.organization Department of Bacteriology and Immunology
dc.contributor.organization Hematologian yksikkö
dc.contributor.organization Department of Medicine
dc.contributor.organization Clinicum
dc.contributor.organization Department of Oncology
dc.contributor.organization Departments of Faculty of Veterinary Medicine
dc.contributor.organization Mikael Skurnik / Principal Investigator
dc.contributor.organization Reetta Maria Satokari / Principal Investigator
dc.contributor.organization Airi Palva / Principal Investigator
dc.contributor.organization Willem Meindert Vos de / Principal Investigator
dc.contributor.organization de Vos & Salonen group
dc.contributor.organization HUS Comprehensive Cancer Center
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.1371/journal.pone.0173004
dc.relation.issn 1932-6203
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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