Changes in plasma protein levels as an early indication of a bloodstream infection

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Kuusela , P I , Saraswat , M , Joenväärä , S , Kaartinen , J , Järvinen , A & Renkonen , R 2017 , ' Changes in plasma protein levels as an early indication of a bloodstream infection ' , PLoS One , vol. 12 , no. 2 , 0172987 . https://doi.org/10.1371/journal.pone.0172987

Title: Changes in plasma protein levels as an early indication of a bloodstream infection
Author: Kuusela, Pentti I; Saraswat, Mayank; Joenväärä, Sakari; Kaartinen, Johanna; Järvinen, Asko; Renkonen, Risto
Other contributor: University of Helsinki, HUSLAB
University of Helsinki, Transplantation Laboratory
University of Helsinki, HUSLAB
University of Helsinki, HUS Emergency Medicine and Services
University of Helsinki, Department of Medicine
University of Helsinki, HUSLAB









Date: 2017-02-24
Language: eng
Number of pages: 17
Belongs to series: PLoS One
ISSN: 1932-6203
DOI: https://doi.org/10.1371/journal.pone.0172987
URI: http://hdl.handle.net/10138/178738
Abstract: Blood culture is the primary diagnostic test performed in a suspicion of bloodstream infection to detect the presence of microorganisms and direct the treatment. However, blood culture is slow and time consuming method to detect blood stream infections or separate septic and/or bacteremic patients from others with less serious febrile disease. Plasma proteomics, despite its challenges, remains an important source for early biomarkers for systemic diseases and might show changes before direct evidence from bacteria can be obtained. We have performed a plasma proteomic analysis, simultaneously at the time of blood culture sampling from ten blood culture positive and ten blood culture negative patients, and quantified 172 proteins with two or more unique peptides. Principal components analysis, Orthogonal Projections to Latent Structures Discriminant Analysis (OPLS-DA) and ROC curve analysis were performed to select protein(s) features which can classify the two groups of samples. We propose a number of candidates which qualify as potential biomarkers to select the blood culture positive cases from negative ones. Pathway analysis by two methods revealed complement activation, phagocytosis pathway and alterations in lipid metabolism as enriched pathways which are relevant for the condition. Data are available via ProteomeXchange with identifier PXD005022.
Subject: C-REACTIVE PROTEIN
ALPHA-1-ACID GLYCOPROTEIN
ORGAN FAILURE
SEPTIC SHOCK
SEPSIS
PROCALCITONIN
BACTEREMIA
CULTURES
DEFINITIONS
EXPRESSION
3111 Biomedicine
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