Prevention of Staphylococcus aureus biofilm formation by antibiotics in 96-Microtiter Well Plates and Drip Flow Reactors : critical factors influencing outcomes

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http://hdl.handle.net/10138/178859

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Manner , S , Goeres , D M , Skogman , M , Vuorela , P & Fallarero , A 2017 , ' Prevention of Staphylococcus aureus biofilm formation by antibiotics in 96-Microtiter Well Plates and Drip Flow Reactors : critical factors influencing outcomes ' , Scientific Reports , vol. 7 , 43854 . https://doi.org/10.1038/srep43854

Title: Prevention of Staphylococcus aureus biofilm formation by antibiotics in 96-Microtiter Well Plates and Drip Flow Reactors : critical factors influencing outcomes
Author: Manner, Suvi; Goeres, Darla M.; Skogman, Malena; Vuorela, Pia; Fallarero, Adyary
Contributor: University of Helsinki, Division of Pharmaceutical Biosciences
University of Helsinki, Division of Pharmaceutical Biosciences
University of Helsinki, Pharmaceutical Design and Discovery group
Date: 2017-03-02
Language: eng
Number of pages: 10
Belongs to series: Scientific Reports
ISSN: 2045-2322
URI: http://hdl.handle.net/10138/178859
Abstract: Biofilm formation leads to the failure of antimicrobial therapy. Thus, biofilm prevention is a desirable goal of antimicrobial research. In this study, the efficacy of antibiotics (doxycycline, oxacillin and rifampicin) in preventing Staphylococcus aureus biofilms was investigated using Microtiter Well Plates (MWP) and Drip Flow Reactors (DFR), two models characterized by the absence and the presence of a continuous flow of nutrients, respectively. Planktonic culture of S. aureus was exposed to antibiotics for one hour followed by 24 hours incubation with fresh nutrients in MWP or continuous flow of nutrients in DFR. The DFR grown biofilms were significantly more tolerant to the antibiotics than those grown in MWP without the continuous flow. The differences in log reductions (LR) between the two models could not be attributed to differences in the cell density, the planktonic inoculum concentration or the surface-area-to-volume ratios. However, eliminating the flow in the DFR significantly restored the antibiotic susceptibility. These findings demonstrate the importance of considering differences between experimental conditions in different model systems, particularly the flow of nutrients, when performing anti-biofilm efficacy evaluations. Biofilm antibiotic efficacy studies should be assessed using various models and more importantly, in a model mimicking conditions of its clinical application.
Subject: IN-VITRO
BACTERIAL BIOFILMS
PSEUDOMONAS-FLUORESCENS
MEDICAL DEVICES
MODEL
EFFICACY
INFECTIONS
REMOVAL
CLARITHROMYCIN
MICROORGANISMS
1182 Biochemistry, cell and molecular biology
317 Pharmacy
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