Effects of Articular Cartilage Constituents on Phosphotungstic Acid Enhanced Micro-Computed Tomography

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http://hdl.handle.net/10138/178861

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Karhula , S S , Finnilä , M A , Lammi , M J , Ylärinne , J H , Kauppinen , S , Rieppo , L , Pritzker , K P H , Nieminen , H J & Saarakkala , S 2017 , ' Effects of Articular Cartilage Constituents on Phosphotungstic Acid Enhanced Micro-Computed Tomography ' PLoS One , vol. 12 , no. 1 , 0171075 . DOI: 10.1371/journal.pone.0171075

Title: Effects of Articular Cartilage Constituents on Phosphotungstic Acid Enhanced Micro-Computed Tomography
Author: Karhula, Sakari S.; Finnilä, Mikko A.; Lammi, Mikko J.; Ylärinne, Janne H.; Kauppinen, Sami; Rieppo, Lassi; Pritzker, Kenneth P. H.; Nieminen, Heikki J.; Saarakkala, Simo
Other contributor: University of Helsinki, Doc­toral Pro­gramme in Ma­ter­i­als Research and Nanos­ciences
Date: 2017-01-30
Language: eng
Number of pages: 12
Belongs to series: PLoS One
ISSN: 1932-6203
DOI: https://doi.org/10.1371/journal.pone.0171075
URI: http://hdl.handle.net/10138/178861
Abstract: Contrast-enhanced micro-computed tomography (CE mu CT) with phosphotungstic acid (PTA) has shown potential for detecting collagen distribution of articular cartilage. However, the selectivity of the PTA staining to articular cartilage constituents remains to be elucidated. The aim of this study was to investigate the dependence of PTA for the collagen content in bovine articular cartilage. Adjacent bovine articular cartilage samples were treated with chondroitinase ABC and collagenase to degrade the proteoglycan and the collagen constituents in articular cartilage, respectively. Enzymatically degraded samples were compared to the untreated samples using CE mu CT and reference methods, such as Fourier-transform infrared imaging. Decrease in the X-ray attenuation of PTA in articular cartilage and collagen content was observed in cartilage depth of 0-13% and deeper in tissue after collagen degradation. Increase in the X-ray attenuation of PTA was observed in the cartilage depth of 13- 39% after proteoglycan degradation. The X-ray attenuation of PTA-labelled articular cartilage in CE mu CT is associated mainly with collagen content but the proteoglycans have a minor effect on the X-ray attenuation of the PTA-labelled articular cartilage. In conclusion, the PTA labeling provides a feasible CE mu CT method for 3D characterization of articular cartilage.
Subject: CT ARTHROGRAPHY
MATRIX METALLOPROTEINASES
GLYCOSAMINOGLYCAN CONTENT
OSTEOARTHRITIC CARTILAGE
COLLAGEN
QUANTITATION
DOMAIN
CLEAVE
SITES
BONE
3111 Biomedicine
114 Physical sciences
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