Co-infecting Reptarenaviruses Can Be Vertically Transmitted in Boa Constrictor

Show full item record



Permalink

http://hdl.handle.net/10138/178867

Citation

Keller , S , Hetzel , U , Sironen , T , Korzyukov , Y , Vapalahti , O , Kipar , A & Hepojoki , J 2017 , ' Co-infecting Reptarenaviruses Can Be Vertically Transmitted in Boa Constrictor ' , PLoS Pathogens , vol. 13 , no. 1 , 1006179 . https://doi.org/10.1371/journal.ppat.1006179

Title: Co-infecting Reptarenaviruses Can Be Vertically Transmitted in Boa Constrictor
Author: Keller, Saskia; Hetzel, Udo; Sironen, Tarja; Korzyukov, Yegor; Vapalahti, Olli; Kipar, Anja; Hepojoki, Jussi
Other contributor: University of Helsinki, Departments of Faculty of Veterinary Medicine
University of Helsinki, Medicum
University of Helsinki, Medicum
University of Helsinki, Medicum
University of Helsinki, Departments of Faculty of Veterinary Medicine
University of Helsinki, Medicum









Date: 2017-01
Language: eng
Number of pages: 21
Belongs to series: PLoS Pathogens
ISSN: 1553-7366
DOI: https://doi.org/10.1371/journal.ppat.1006179
URI: http://hdl.handle.net/10138/178867
Abstract: Boid inclusion body disease (BIBD) is an often fatal disease affecting mainly constrictor snakes. BIBD has been associated with infection, and more recently with coinfection, by various reptarenavirus species (family Arenaviridae). Thus far BIBD has only been reported in captive snakes, and neither the incubation period nor the route of transmission are known. Herein we provide strong evidence that co-infecting reptarenavirus species can be vertically transmitted in Boa constrictor. In total we examined five B. constrictor clutches with offspring ranging in age from embryos over perinatal abortions to juveniles. The mother and/or father of each clutch were initially diagnosed with BIBD andor reptarenavirus infection by detection of the pathognomonic inclusion bodies (IB) andor reptarenaviral RNA. By applying next-generation sequencing and de novo sequence assembly we determined the "reptarenavirome " of each clutch, yielding several nearly complete L and S segments of multiple reptarenaviruses. We further confirmed vertical transmission of the co-infecting reptarenaviruses by species-specific RT-PCR from samples of parental animals and offspring. Curiously, not all offspring obtained the full parental "reptarenavirome". We extended our findings by an in vitro approach; cell cultures derived from embryonal samples rapidly developed IB and promoted replication of some or all parental viruses. In the tissues of embryos and perinatal abortions, viral antigen was sometimes detected, but IB were consistently seen only in the juvenile snakes from the age of 2 mo onwards. In addition to demonstrating vertical transmission of multiple species, our results also indicate that reptarenavirus infection induces BIBD over time in the offspring.
Subject: INCLUSION-BODY DISEASE
UPDATED PHYLOGENETIC ANALYSIS
BOID SNAKES
ARENAVIRUSES
TRANSMISSION
ALIGNMENT
PYTHONS
VIRUS
IDENTIFICATION
REPLICATION
1183 Plant biology, microbiology, virology
Rights:


Files in this item

Total number of downloads: Loading...

Files Size Format View
journal.ppat.1006179.pdf 2.060Mb PDF View/Open

This item appears in the following Collection(s)

Show full item record