PP2A-B’γ modulates foliar trans-methylation capacity and the formation of 4-methoxy-indol-3-yl-methyl glucosinolate in Arabidopsis leaves

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Rahikainen , M , Trotta , A , Alegre , S , Pascual , J , Vuorinen , K , Overmyer , K , Moffatt , B , Ravanel , S , Glawischnig , E & Kangasjarvi , S 2017 , ' PP2A-B’γ modulates foliar trans-methylation capacity and the formation of 4-methoxy-indol-3-yl-methyl glucosinolate in Arabidopsis leaves ' , Plant Journal , vol. 89 , no. 1 , pp. 112-127 . https://doi.org/10.1111/tpj.13326

Title: PP2A-B’γ modulates foliar trans-methylation capacity and the formation of 4-methoxy-indol-3-yl-methyl glucosinolate in Arabidopsis leaves
Author: Rahikainen, Moona; Trotta, Andrea; Alegre, Sara; Pascual, Jesus; Vuorinen, Katariina; Overmyer, Kirk; Moffatt, Barbara; Ravanel, Stephane; Glawischnig, Erich; Kangasjarvi, Saijaliisa
Contributor: University of Helsinki, University of Turku
University of Helsinki, Plant-Fungal Interactions Group
University of Helsinki, Biosciences
University of Helsinki, University of Turku
Date: 2017-01
Language: eng
Number of pages: 16
Belongs to series: Plant Journal
ISSN: 0960-7412
URI: http://hdl.handle.net/10138/178883
Abstract: Glucosinolates (GSL) of cruciferous plants comprise a major group of structurally diverse secondary compounds which act as deterrents against aphids and microbial pathogens and have large commercial and ecological impacts. While the transcriptional regulation governing the biosynthesis and modification of GSL is now relatively well understood, post-translational regulatory components that specifically determine the structural variation of indole glucosinolates have not been reported. We show that the cytoplasmic protein phosphatase 2A regulatory subunit B'gamma (PP2A-B'gamma) physically interacts with indole glucosinolate methyltransferases and controls the methoxylation of indole glucosinolates and the formation of 4-meth-oxy-indol-3-yl-methyl glucosinolate in Arabidopsis leaves. By taking advantage of proteomic approaches and metabolic analysis we further demonstrate that PP2A-B'gamma is required to control the abundance of oligomeric protein complexes functionally linked with the activated methyl cycle and the trans-methylation capacity of leaf cells. These findings highlight the key regulatory role of PP2A-B'gamma in methionine metabolism and provide a previously unrecognized perspective for metabolic engineering of glucosinolate metabolism in cruciferous plants.
Subject: protein phosphatase 2A
glucosinolate
activated methyl cycle
Arabidopsis thaliana
L-HOMOCYSTEINE HYDROLASE
CYSTATHIONINE GAMMA-SYNTHASE
INDOLE-GLUCOSINOLATE
PLANT-CELLS
METHYLTRANSFERASE ACTIVITY
S-ADENOSYLHOMOCYSTEINE
METHIONINE METABOLISM
PROTEIN INTERACTIONS
CRYSTAL-STRUCTURES
STRESS RESPONSES
1183 Plant biology, microbiology, virology
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