Recent Submissions

  • Korkiakangas, Timo (Helsingin yliopisto, 2016)
    The object of this study is the case marking of the subject in early medieval charter Latin. The work explores whether and how the nominative/accusative-type morphosyntactic alignment changed into a semantically motivated (active/inactive) alignment in Late Latin before the disappearance of the case system. It is known that the accusative originally the case of the direct object extended in Late Latin to the subject function in which Classical Latin allowed only the nominative. On this basis, it has been postulated that in Late Latin the nominative/accusative contrast was (re)semanticized so that the nominative came to encode all the Agent-like arguments and the accusative all the Patient-like arguments. The study examines which semantic and syntactic factors determine the selection of the subject case in each subject/finite verb combination in the Late Latin Charter Treebank (LLCT). The LLCT is an annotated corpus of Latin charter texts (c. 200,000 words) written in Tuscany between AD 714 and 869. The central result of the study is that the Latin of the LLCT shows a semantically based morphosyntactic alignment in those parts of nominal declension where the morphological contrast between nominative- and accusative-based forms is morphophonologically intact. The following picture of intransitivity split turns up: the low-animacy subjects of the LLCT occur more often in the accusative than do the agentive high-animacy subjects. Likewise, the accusative percentage of SO subject constructions is higher than that of A/SA subject constructions. The common denominator of the examined semantic variables is likely to be the control exercised by the subject over the verbal process. Syntactic factors seem to influence the case distribution pattern as well. For example, the immediate preverbal position of the subject implies a high retention of the nominative. The immediate preverbal position of SV(O) language is a canonical subject position where the syntactic complexity measured as dependency lengths is at its lowest and the cohesion of the verbal nucleus at its highest. Thus, a by then already marked nominative form results. The control of the subject over the verbal process (semantic variable) and the cohesion of the verbal nucleus (syntactic variable) may be partly conflated, i.e., both may affect the subject case selection in certain conditions.
  • Kauppinen, Saara (Helsingin yliopisto, 2015)
    This study discusses the language, especially the communicative structures, of Greek dialogue epigrams. The central research questions include: Who are the speakers? How are they identified? How does the recipient know when the speaker changes? How is the turn-division implied? What are the functions of the speakers? In the study, all the dialogue verse inscriptions are collected and divided according to typology that was created for this study and which is based on the pair structure. The most common adjacency pair of dialogue epigrams is a question answer pair, and many of the elements studied here are features of question structures, but other pairs, such as greeting pairs, are also taken into account. The dialogues contain either one adjacency pair (type 1), several of them (type 2) or longer, often narrative units that form pair-like structures (type 3). The types, their variants and features of the language characteristic of each are discussed using examples. Elements such as addresses, imperatives and particles are central to my argument, and speaker roles and pair structure variants are also discussed. Non-inscriptional epigrams are given as parallels for each type, and the mutual influence between them and the verse inscriptions is discussed using examples. The reception situation and possible performance of the epigrams is also discussed, and on the basis of various examples, it is stated that the representation of the speakers often implies the complex reception situation, part of which was most probably reading the epigram aloud. Epigraphic methods are used and combined, and the linguistic aspect brings a new perspective to epigraphic studies. The comparison of the verse inscriptions and the literary ones contributes to ongoing discussions on the epigram genre and its inscriptional counterparts. The material discussed is mainly from the Roman period, a phase less well discussed in recent epigram studies, and the study thus adds to our knowledge of the genre and the mutual influence between the post-Hellenistic verse inscriptions and non-inscriptional epigrams.
  • Vogt, Robert (Helsingin yliopisto, 2015)
    Theory and Typology of Narrative Unreliability (Theorie und Typologie narrativer Unzuverlässigkeit) „The theory of unreliable narration is obviously one of the current boom-sectors of what Manfred Jahn and Ansgar Nünning have called the ‘narratological industry’“, Tom Kindt (2008: 129) writes. In a similar vein, D’hoker and Martens (2008: 2) detect „an almost uncanny centrality and importance“of the concept in the field of narratology. Indeed, debates concerning the categorization of different forms of unreliable narration, the scope of the concept and explanation models based on different approaches enjoy wide currency. In the study, I aim to develop a theory and typology of narrative unreliability. Following McHale (1981: 185) that “[b]efore a phenomenon can be explained it must first exist for those who would explain it, which means that it must be constituted as a category with boundaries and a name“, chapter II establishes a typology of narrative unreliability. Claiming that the concept of unreliability is used in different ways in narratology, I distinguish three basic forms. First, unreliability can be understood as a trait of a homodiegetic narrator or a focalizer which manifests as a questionable account or interpretation of the events in the narrative world (ironic unreliability). Second, unreliability can be regarded as a feature of the narrative discourse which leaves open whether or not a narrator or a focalizer depicts or evaluates the narrative world in an adequate way (ambiguous unreliability). Third, unreliability can designate a feature of the narrative discourse which leads the reader astray about the actual events in the fictional world (alterated unreliability). Having defined the field of inquiry, chapter III sets out to develop a model to describe and explain these different forms of narrative unreliability. After addressing some of the theoretical stumbling blocks narratologists face when theorizing narrative unreliability in fictional works, chapters IV and V I outline how insights from literary possible-worlds and cognitive narratology might help to develop a better understanding of how readers construct fictional universes in texts featuring ironic, ambiguous, and alterated unreliability. According to literary possible-worlds theory, fictional works create their own modal system of reality which consists of a multitude of worlds. In the center of each fictional universe is the so-called ‘textual actual world’ (TAW) which is surrounded by the different characters’, focalizer’s or narrator’s mental worlds. Within this theoretical framework, narrative unreliability occurs in literary works when the narrator’s or focalizer’s mental world is in conflict with TAW. As the relations between different worlds within a fictional universe are the result of interpretative processes, I argue that the main difference between the three forms of narrative unreliability lies in the way a reader makes sense of world conflicts: how he distinguishes different worlds, how he detects conflicting worlds and how he puts conflicting worlds in a hierarchical order to determine what is fact in the fictional universe. Since „possible-world approaches do not ultimately treat fictional worlds as cognitive products and do not deal with cognitive processing“ (Semino 2003: 89), chapter V addresses the question of how a reader constructs fictional universes in unreliable narratives. By recourse to insights from cognitive science – including approaches belonging to the “information processing paradigm” as well as those belonging to the mental “disposition paradigm” – I explain how readers distinguish different worlds, detect conflicting worlds and put these into a hierarchical order. In order to illustrate the scope and variety of narrative unreliability, I discuss six British and American literary works in chapters VI to VIII. Chapter VI focuses on ironic unreliability in Charles Dickens’ David Copperfield and in Kazuo Ishiguro’s The Remains of the Day. Chapter VII analyses ambiguous unreliability in Ford Madox Ford’s The Good Soldier and in Bret Easton Ellis’ American Psycho. Ambrose Bierce’s “An Occurrence at Owl Creek Bridge” and Chuck Palahniuk’s Fight Club serve as examples of alterated unreliable narration (chapter VIII). In chapter IX, I discuss the metacognitive dimension of narrative unreliability. Assuming that „[narratives] operat[e] as an instrument of mind in the construction of reality“ (Bruner 1991: 6), I claim that narrative unreliability highlights and reflects cognitive processing of a narrator, of a focalizer, or of a reader in different ways. While ironic unreliability puts the emphasis on the idiosyncratic ways of world-making of a cognitive center (either of a homodiegetic narrator or of a focalizer), alterated unreliability with its surprising plot twist stimulates readers to go backwards and monitor how he or she was led down a garden path. I analyse Ian McEwan’s Atonement to demonstrate that the characters’ (mistaken) attempts to construct reality (ironic-unreliable focalization) is mirrored by the reader’s mistaken reconstruction of the facts and events in the fictional world (alterated-unreliable narration). The study concludes with a summary of the most important insights and a brief reflection on how the theory can be applied to other media such as film, comics or video games (chapter X).
  • Kivikangas, J Matias (Helsingin yliopisto, 2015)
    Arguably, the emotions elicited by playing are the reason why people play digital games. Social interaction is an important source of emotion during game play, but research on it is rather sparse. In this dissertation I briefly review the emotion-theoretic literature in order to better understand what emotion means in the context of games, and how this should be taken into account when measuring emotions related to a game experience. Study I presents a review of the use of psychophysiological methods in game research. I show that the theoretical background behind these methods generally tends to be neglected. This could be remedied by a theoretical framework that integrates the understanding of emotions and explicitly describes the links between different emotion measures and the theoretical concepts they are professed to reflect. I present my proposition for the first step towards such a framework in Study II. I employ the sociality characteristics framework by de Kort and IJsselsteijn (2008) and my interpretation of the social factors in order to study the effect of the central social context factors on the emotional game experience. Study III presents evidence that in addition to tonic physiological levels, the relationship between the participants also affects the momentary, phasic responses to the key game events victory and defeat. In particular, although physiological signals can, to a certain extent, be used to assess emotional experiences (such as positive responses to a victory), in some cases the typical psychophysiological mappings may even be completely opposite. Interpreting these signals requires a broader theoretical understanding than what is typically acknowledged. Study IV supports the earlier findings that competition is experienced more positively than cooperation but that the effect is dependent on gender, as this was found only in males. For females, there was no difference between the two modes, and no difference in negative activation. In addition, self-reports concerning social presence suggested that this concept is not always associated with higher positive emotions, while a form of friendly rivalry (associated with lower social presence) might be experienced positively a finding apparently new in existing literature. Finally, Study V provides insight into the practical significance of the measurements with a predictive validity study, showing practical effects how the certain kinds of game experiences may lead to greater game use and preference, but that these links are not as simple as previously suggested. In sum, this work offers new knowledge on how social context factors are generally related to the game experience, on how emotions can be studied in game research and what theoretical considerations should be taken into account, and on the emotional effects of particular social context factors during play. The results are mainly useful for further basic game research, but they have also potential implications for general emotion research, the game industry, and in the long run, society at large.
  • Arola, Teppo (Helsingin yliopisto, 2015)
    Increase of greenhouse gas concentrations in the atmosphere, the limits of conventional energy reservoirs and the instability risks related to energy transport have forced nations to promote the utilisation of renewable energy reservoirs. Groundwater can be seen as an option for renewable energy utilisation and not only a source of individual or municipal drinking water. Finland has multiple groundwater reservoirs that are easily exploitable, but groundwater energy is not commonly used for renewable energy production. The purpose of this thesis study was to explore the groundwater energy potential in Finland, a region with low temperature groundwater. Cases at three different scales were investigated to provide a reliable assessment of the groundwater energy potential in Finland. Firstly, the national groundwater energy potential was mapped for aquifers classified for water supply purposes that are under urban or industrial land use. Secondly, the urbanisation effect on the peak heating and peak cooling power of groundwater was investigated for three Finnish cities, and finally, the long-term groundwater energy potential was modelled for 20 detached houses, 3 apartment buildings and a shopping centre. The thesis connects scientific information on hydro- and thermogeology with the energy efficiency of buildings to produce accurate information concerning groundwater energy utilisation. Hydrological and thermogeological data were used together with accurate data on the energy demands of buildings. The heating and cooling power of groundwater was estimated based on the groundwater flux, temperature and heat capacity and the efficiency of the heat transfer system. The power producible from groundwater was compared with the heating and cooling demands of buildings to calculate the concrete groundwater energy potential. Approximately 20% to 40% of annually constructed residential buildings could be heated utilising groundwater from classified aquifers that already are under urban land use in Finland. These aquifers contain approximately 40 to 45 MW of heating power. In total, 55 to 60 MW of heat load could be utilised with heat pumps. Urbanisation increases the heating energy potential of groundwater. This is due anthropogenic heat flux to the subsurface, which increases the groundwater temperatures in urbanised areas. The average groundwater temperature was 3 to 4 °C higher in city centres than in rural areas. Approximately 50% to 60% more peak heating power could be utilised from urbanised compared with rural areas. Groundwater maintained its long term heating and cooling potential during 50 years of modelled operation in an area where the natural groundwater temperature is 4.9 °C. Long-term energy utilisation created a cold groundwater plume downstream, in which the temperature decreased by 1 to 2.5 °C within a distance of 300 m from the site. Our results demonstrate that groundwater can be effectively utilised down to a temperature of 4 °C. Groundwater can form a significant local renewable energy resource in Finland. It is important to recognise and utilise all renewable energy reservoirs to achieve the internationally binding climatological targets of the country. Groundwater energy utilisation should be noted as one easily exploitable option to increase the use of renewable energy resources in a region where the natural groundwater temperature is low. The methods presented in this thesis can be applied when mapping and designing groundwater energy systems in nationwide- to property-scale projects. Accurate information on hydro- and thermogeology together with the energy demands of buildings is essential for successful system operation.
  • Weiste, Elina (Helsingin yliopisto, 2015)
    The quality of the therapeutic relationship is highly significant for treatment outcomes in mental healthcare. While the value of the relationship has been clearly documented, the various aspects of how the relationship is actualized in clinical practice have remained unclear. This dissertation breaks new ground in understanding how the therapeutic relationship is manifested in three forms of therapeutic interaction: psychoanalysis, cognitive psychotherapy and resource-centred counselling. The method of conversation analysis is applied to compare these approaches and reveal how specific aspects of the therapeutic relationship are managed in interaction: 1) how therapists express empathy and respond to clients talk on their subjective emotional experiences, 2) how therapists work with experiences that belong to clients personal domains of knowledge, and 3) how disagreements are expressed and relational stress managed in therapeutic interaction. The data comprise audio- and video-recorded encounters from each therapeutic approach (86 encounters in total). The data analysis reveals the fine-grained interactional practices used in the management of the therapeutic relationship. In all the therapeutic approaches, formulating the client s emotional experience allowed the therapists to display empathic understanding, and prosodic features were important for marking the formulation as either empathic or challenging. In psychoanalysis and cognitive psychotherapy, the client s emotional experiences were typically validated, interpreted or challenged. In the resource-centred approach, the clinicians sought to focus on successful experiences and praised clients agency and competence, while shifting the focus away from their difficult emotional experiences. The data analysis also highlights the complex relationship between emotions and epistemics and describes how a delicate balance between empathic and challenging interventions is manifested in therapists supportive and unsupportive moves during extended disagreement sequences. This dissertation contributes to three areas of research: 1) clinical research, as it underlines the importance of investigating the actions of the therapist and client in a relational way, furthering comprehension of how the processes associated with the therapeutic relationship appear in the context of interaction between therapist and client; 2) sociological studies on mental health, as this study illustrates some important institutional differences between psychotherapy and psychiatric outpatient care; 3) conversation analysis, as this research provides the first broader systematic comparison of interactional practices in different therapeutic approaches.
  • Mustonen, Eeva (Helsingin yliopisto, 2015)
    Forage legumes such as red clover (Trifolium pratense L.) are used due to their ability to fix atmospheric nitrogen. Red clover has a high crude protein content and sufficient digestible fibre. Red clover silage tends to increase dry matter intake and milk yield in dairy cows. The use of clover silage can lead to desirable changes in milk fatty acid composition, promote growth and increase live-weight gain in ewes and lambs. The literature review focuses on the principal isoflavones in red clover, their metabolism in the rumen and conjugation and excretion. The role of equol is reviewed regarding concentrations in plasma and milk. Red clover varieties studied contained isoflavones; formononetin (6.0–7.9 mg/g in dry matter (DM)), biochanin-A (3.7–6.1 mg/g in DM), genistein (0.5-0.6 mg/g in DM) and daidzein (0.2–0.30 mg/g in DM). There were significant differences in isoflavone contents. The isoflavone content was highest in cultivar Ilte, which had the highest concentration of formononetin. The effect of growing conditions (year), growth stage (harvest) and habitat (site) were significant for formononetin concentrations. The total isoflavone and formononetin concentrations were highest under poor weather conditions, at the northern location and with later harvest. In the feeding experiment, the ewes were fed red clover (RC) silage that contained on average 6.8 mg/g of formononetin in DM. Timothy/meadow fescue (Phleum pratense L./Festuca pratensis Huds.) grass silage did not contain any isoflavones. In the RC group, estimated daily intake of isoflavones was 10.5 g formononetin. The major part of formononetin ingested was metabolized, contributing to the increase of equol in the serum (average level of 7.7 mg/l). In HPLC analyses, the serum of ewes contained solely (S)-equol. The ewes of RC group gained weight faster (P<0.001) and were significantly heavier (P<0.01) at the end of the experiment. All ewes became pregnant. There were no significant differences in the time of conception, numbers or weight of foetuses or numbers of ovulations. The mass of the uterus was significantly greater (P<0.01) in the RC group compared with that in the control group. This difference was mainly explained by the increase of foetal fluids in the RC group (P<0.01). In the dairy cow feeding experiment, the RC silages contained 3.0–6.5 mg/g formononetin in DM. Grass silage did not contain any isoflavones. The formononetin contents of the red clover were highest for the shortest growthperiod. The daily intake per cow of daidzein was 1.7–2.6 g and of formononetin 27–76 g. The equol concentration in plasma was 4.6–8.4 mg/l. Intake of formononetin was strongly associated with the equol concentration in plasma (R2 0.71). Equol contents in plasma were significantly higher (P<0.01) for the RC than for the cows fed grass silage. Plasma equol contents were significantly higher (P<0.001) for fed silages, where the growth period were shortest. Equol concentrations in the milk of cows fed RC were 458–643 µg/l and daily secretion of equol in milk was estimated to be 12–19 mg/day. Intake of formononetin was only weakly associated with equol concentration in milk (R2 0.20). Equol concentrations were also analysed from organic and conventionally produced commercial milk samples. Organic skimmed milk contained 411 µg/l of equol and conventionally produced 62 µg/l. The HPLC method provided excellent accuracy and sensitivity for quantification of isoflavonoids from fodder, blood, and milk samples. (S)-equol was the main isoflavonoid detected. It is suggested that the estrogenic effects of metabolic equol in sheep reported earlier are solely or predominantly due to (S)-equol. In Finnish landrace sheep, the fecundity of ewes was not reduced by feeding RC. The volume of foetal fluids, however, increased, which could increase the risk of vaginal prolapse before term. A strong association between formononetin intake and equol concentration in plasma was demonstrated. The equol content in cow’s milk can be as high as 600 µg/l with RC silage feeding, even though only a small part of the formononetin is secreted into milk as the metabolite equol. Milk equol is derived from the formononetin of RC silage and RC-fed cows’ milk can be considered as a source of equol in human nutrition.
  • Keriö, Susanna (Helsingin yliopisto, 2015)
    During their life, conifers are attacked by fungal pathogens and insects. In the European forests, Heterobasidion annosum sensu stricto (s.s.) attacks Scots pine (Pinus sylvestris) roots, whereas Heterobasidion parviporum causes the majority of decay in Norway spruce (Picea abies), both causing severe economic losses. Another significant health problem of Scots pine is caused by Hylobius abietis, the large pine weevil, which damages newly established Scots pine stands. Several defence reactions in trees are activated upon fungal infection and insect attack, but these reactions have not been comprehensively studied in conifers. In this dissertation, the responses of mature Norway spruce and Scots pine trees to Heterobasidion spp. inoculation, and the responses of Scots pine saplings to Hylobius abietis feeding were studied. Also the ability of homokaryotic Heterobasidion spp. isolates to infect mature conifer hosts and elicit defensive responses was investigated. Terpene and transcript profiles of Scots pine to H. annosum s.s. and H. abietis challenge were studied, and a customised oligonucleotide microarray with 36.5K cDNA elements designed based on the P. taeda transcriptome was used to study the Scots pine transcriptome. The used homokaryotic Heterobasidion spp. isolates were able to colonize and evoke defence responses in the host trees with varying levels of susceptibility. Insect feeding and fungal inoculation induced terpene production in Scots pine. The results indicated that high accumulation of terpenes is not necessarily an effective defence against H. annosum, but δ-3-carene might be associated with higher tolerance to H. annosum in Scots pine. Only few genes related to terpene synthesis were induced in response to H. annosum infection and weevil feeding. Induction of genes related to biotic and abiotic stress responses indicated a wide transcriptomic reprogramming in response to fungal infection and weevil feeding. Genes related to signal perception and defence responses were induced especially in the trees less susceptible to H. annosum inoculation. In addition to these genes, Scots pine δ-3-carene synthases are promising candidates for further research on the Scots pine resistance to H. annosum.
  • Kandolin, Riina (Helsingin yliopisto, 2015)
    Cardiac sarcoidosis (CS) and giant cell myocarditis (GCM) are underdiagnosed inflammatory myocardial diseases. Sarcoidosis is a systemic disease characterized by granuloma formation and subsequent tissue scarring in various organs, most commonly in the lungs. In majority of cases, lung sarcoidosis is a self-limiting disease whereas cardiac involvement carries a poorer prognosis due to heart failure and malignant arrhythmias. GCM is a rare, frequently fatal myocardial disease designated by widespread myocardial inflammation and necrosis. Prior data concerning epidemiology, outcome with contemporary treatment and ICDs, and the effect of novel diagnostic methods is scarce and I set out to study these questions. All cases with histologically confirmed CS and GCM in Finland between 1988 and 2014 were identified and analyzed. A marked increase in the detection rate of CS over the last 26 years was found. In the era of modern diagnostic imaging and increased awareness, the annual detection rate of CS is over 50 times higher than before. CS most commonly manifests with atrioventricular block (AV-block). What is more, CS and GCM together explain 25% of initially idiopathic 2nd to 3rd degree AV-blocks in adults aged 18-55 years. Other principal manifestations of CS are heart failure and ventricular arrhythmias. Two thirds of patients with CS present without prior diagnosis of sarcoidosis, an entity called clinically isolated CS. The mean age of CS patients was 51 years and two thirds were female. The diagnosis was based on endomyocardial biopsy (EMB) in 50% of cases and on extracardiac biopsy combined with cardiac imaging findings (cardiac magnetic resonance (CMR) or positron emission tomography (PET)) in 50% cases. Single EMB session had a sensitivity of approximately 30% in detecting CS, but repeated biopsies or taking histologic samples from mediastinal lymph nodes markedly improved the diagnostic yield. In majority of cases, CS is a slowly progressive cardiomyopathy. With up-to-date diagnostic methods and treatment, 99% of patients were alive without cardiac death or transplantation at 1 year and 91% after 10 years from symptom onset. The most common presentations in GCM patients were heart failure and AV-block. Moreover, ventricular arrhythmias were common with two third of patients experiencing sustained ventricular tachycardia or ventricular fibrillation during the disease course. With current diagnostic methods and therapy with a combination of immunosuppressants, the transplant free survival was 69% at 1 year and 52% at 5 years. In conclusion, the detection rate of CS and GCM in Finland is increasing and the prognosis with contemporary diagnostic and therapeutic methods seems better than previously reported.
  • Puranen, Taija (Helsingin yliopisto, 2015)
    Nutritional problems such as poor appetite and unintentional weight loss are common among individuals with Alzheimer’s disease (AD), and their older spousal caregivers with comorbidities may also be at risk of malnutrition. The objective was to study the nutrition of people with AD and their spouses, and to compare nutrient intake to recommended levels, and to investigate the association between the caregiver’s gender and the couples’ nutrition. A randomized, controlled trial was conducted to examine the effectiveness of nutritional guidance on the AD victim’s weight, nutritional status (MNA), energy and nutrient intake (three-day food diaries), quality of life (HRQoL with 15D) and falls. A total of 99 couples were randomized into this trial. The intervention group (IG) received tailored nutritional guidance 4-8 times at their homes over one year, and the control group (CG) received booklet on healthy nutrition. The primary outcome measure was the AD sufferer’s weight change. The mean age of AD was 77.4 years (SD 5.6), and spouses 75.2 years (SD 7.0). At baseline, 44% of the AD, and 16% of the spouses were at risk of malnutrition, whereas 56% and 84% had a good nutritional status. At baseline, the mean energy intake among those with AD was 1897 kcal (SD 416) among the men and 1313 kcal (SD 340) among the women, and the respective figures for spousal caregivers were 1605 kcal (SD 458) and 1536 kcal (SD 402). Among AD, 47% of the men and 71% of the women had a protein intake below 1g /bodyweight/kg, the respective figures for the spouses being 71 and 49%. In addition, almost half of the participants had a vitamin C intake of less than the recommended. The male gender of the caregiver was associated with poor energy and nutrient intake in the couple. Tailored nutritional guidance had no effect on the weight of those with AD, but improved their nutrient intake and HRQoL. The mean change in protein intake was 0.05 g per body/kg in kg (95% CI -0.06 to 0.15) in the IG, and -0.06 g per body/kg in the CG (95% CI -0.12 to 0.02), p = 0.031. The respective changes in mean calcium intake were 85 mg (95% CI -24 to 194) and -17 mg (95% CI -98 to 65), p = 0.025. HRQoL improved by 0.006 (95% CI -0.016 to 0.028) in the IG, and declined by -0.036 (95% CI -0.059 to 0.013) in the CG, p = 0.007. In addition, those in the IG with AD had a significantly lower number of falls than those in the CG during the one year: 0.55 (95% 0.34 to 0.83) and 1.39 (95% CI 1.04 to 1.82) falls per person, p < 0.001, respectively. The community-dwelling ID sufferers and their spousal caregivers were very heterogeneous in terms of nutrition. Male caregivers may need tailored guidance on food-related activities. Tailored nutritional guidance improves both nutrition and the quality of life among those with AD, and may also prevent falls, and should therefore be part of their everyday care.
  • Quintero, Ileana B. (Helsingin yliopisto, 2015)
    Prostatic acid phosphatase (PAP) has been linked to prostate cancer since the mid-1930s. The main research approach of PAP over that time has been based on its role in the human prostate. The regulatory mechanisms of expression of the PAP gene have also been studied, giving us information about the regulatory elements in the gene and the transcription factors that affect the gene expression in the prostatic tissue. However, little was known until recently about this protein s role and physiological function in other tissues. Our group generated and used a PAP-deficient mouse and was able to show that PAP is expressed in dorsal root ganglia (DRG) and spinal cord in mice. This is the same protein as thiamine monophosphatase (TMPase) whose enzymatic activity has been used for five decades to mark primary sensory neurons. In these tissues, PAP acts as an ecto-5'-nucleotidase and is able to dephosphorylate AMP to adenosine, and therefore produce an anti-nociceptive effect due to the binding of adenosine to the A1-adenosine receptor. We analyzed the ACPP gene, which enabled us to describe a new transmembrane isoform for PAP (TMPAP). This novel PAP isoform is produced by alternative splicing of the 11th exon of the ACPP gene. The alternative splicing is present in species such as the human, mouse and rat. The newly discovered isoform is widely expressed in human and mouse tissues and contains a tyrosine sequence (YxxΦ) in its carboxyl-terminus, which directs the protein to endocytosis. We have also corroborated its functionality by co-localization studies with different organelle markers in the endosomal/lysosomal pathway (I). The generation of a PAP-deficient mouse also enabled us to study the function/s of PAP in vivo. The lack of PAP in this mouse model led to the gradual changes in the mouse prostate that finally culminated with the development of prostate adenocarcinoma at the age of 12 months. Microarray analyses of different tissues that compared the PAP deficient mouse with the wild type (WT) mouse revealed changes in genes related to the vesicular trafficking, which support our previous results and led us to the conclusion that TMPAP could be involved in the regulation of the vesicular trafficking. We also detected the interaction between TMPAP and snapin, a SNARE (Soluble NSF Attachment Protein Receptor) associated protein, by yeast two-hybrid screening, co-localization and FRET (Förster resonance energy transfer) analysis. We concluded that, the disruption of this interaction in the PAP-deficient mouse leads to a disturbance in the vesicular transport of the cell and to the development of prostate adenocarcinoma in the PAP-deficient mouse prostate (II). Furthermore, we showed that the PAP-deficient mice present multiple behavioral and neurochemical alterations including increased size of brain lateral ventricles, hyperdopaminergic deregulation, and altered GABAergic transmission, symptoms that indicate that PAP also disturbes the normal function of the central nervous system (III). Snapin protein in the brain has been described as a protein important for the vesicular transport and for the fusion of vesicles with the plasma membrane, and we observed that the lack of PAP in GABAergic neurons leads to a change in the localization of snapin in the PAP-deficient mouse (III), which could indicate that as in the prostate a dysregulated vesicular trafficking could be the reason for the detected phenotype. The discovery of the new TMPAP and its localization in the endosomal/lysosomal pathway enabled an understanding of the phenotypic changes that occur in the PAP-deficient mouse. We hypothesized that TMPAP regulates vesicular trafficking by interacting with snapin, and its deficiency leads to a dysregulation of the endo-/exocytosis cycle, which produces the observed alterations in the mouse organs and tissues. The results obtained throughout this research project have opened up new lines of research related to PAP physiological function, and a deeper understanding of the expression, regulation and function of this protein could lead to new clinical applications.
  • Ylivuori, Soile (2015)
    My doctoral dissertation examines the complex relationship of gender construction and politeness in eighteenth-century England. It contributes to a vibrant field of historical research, examining politeness as an intellectual and cultural construct that was used to create individual and group identity. The study combines intellectual and cultural-historical methods with poststructuralist gender studies; through this interdisciplinary methodology, my goal is to introduce a novel approach to the historical research of politeness traditionally reluctant to utilise theoretical apparatuses as an aid of analysis and to suggest that such methods provide fruitful new readings of politeness and its intersection with gender, thus opening up new areas of research. The dissertation is divided into two parts. In the first part, I analyse politeness as a disciplinary practice that produced polite femininity defined in terms of softness, gracefulness, and modesty by regulating the movements and appearances of individuals bodies. This analysis is based on a wide selection of printed source material, such as conduct books, periodicals, sermons, and novels. My main argument is that the female body had a central role in the construction of normative polite femininity, both on a discursive and an individual level. Women of the social elite were urged to internalise a gendered polite identity by exercising and disciplining their bodies to meet the norms of polite femininity deemed natural despite the fact that within the heterogeneous politeness discourse, there was no consensus on what these natural norms exactly were. Moreover, I want to suggest that the ambiguous position of the body as both the means through which an identity is produced and worked on, as well as the allegedly truthful and unerring indicator of an individual s level of polite ideality created a fundamental conflict within the culture of politeness, forcing women into hypocritical positions in practice while simultaneously advocating honesty as the essential emblem of femininity. However, seeing politeness solely as a disciplinary regime provides a one-sided understanding of politeness, since it ignores individual subjectivity. Therefore, the second part of my dissertation examines the journals and letters of four eighteenth-century elite women Catherine Talbot, Mary Delany, Elizabeth Montagu, and Fanny Burney and looks at how these women dealt with the discursive ideals and demands imposed upon them. I argue that individuals had a complex relationship with discursive ideality, and that politeness was not solely a disciplinary regime that lorded over women s behaviour and identity. The profound heterogeneity of the culture of politeness gave, in itself, individuals some freedom of movement within it. More importantly, individuals engaged in specific strategies, or techniques of the self, in order to gain freedom from and within the restrictive norms of polite femininity. These strategies can be seen as clever utilisations of some of the central aspects of politeness with a subversive intent. They concentrate on challenging and redefining the naturalised formulations regarding authenticity, identity, femininity, and politeness, and include such practices as self-discipline, multiplicity of identity, play between exterior and interior, and hypocrisy.
  • Purkamo, Lotta (Helsingin yliopisto, 2015)
    Microbial life in the deep subsurface contributes significantly to overall biomass on Earth. Although the microbial communities inhabiting the deep subsurface are abundant, little is known about their diversity, activity, interactions and role in global biogeochemical cycles. The diversity of microbial life in the deep terrestrial subsurface of the Fennoscandian shield was studied with molecular biological methods. The Outokumpu Deep Drill Hole provides access to crystalline bedrock fluids that are estimated to be tens of millions of years old. Characterization of the indigenous bacterial and archaeal communities in addition to microbial communities with important functional properties in bedrock fluids was done from a depth range of 180 m to 2300 m. Microbial community profiling and assessment of possible functional processes was done with molecular fingerprinting, cloning and sequencing methods combined with suitable statistical and bioinformatics analyses. Low cell numbers but high diversity was characteristic to the microbial communities of the Outokumpu deep subsurface. The microbial communities in the fracture zones had in general fewer cells than those in the mixed fluids of the drill hole. Comamonadaceae, Peptococcaceae and Anaerobrancaceae were prevalent bacterial members of the microbial communities in the fracture fluids. Archaea were a minority in microbial communities. Sulfate-reducing bacteria and methanogens were detected at several depths. Microbial communities resembled those detected from other deep Fennoscandian Shield subsurface sites. Furthermore, sulfate reducing communities and archaeal communities resembled those found from the deep subsurface of South Africa. Investigation on carbon assimilation strategies of the microbial communities revealed that mainly heterotrophic Clostridia were responsible for CO2 fixation in this habitat. Representatives of Burkholderiales and Clostridia formed the core microbial community and these were also identified to be the keystone genera. The microbial communities of Outokumpu fractures share similarity with those of serpentinization-driven ecosystems. Energy and carbon substrates formed in serpentinization reactions of ophiolitic rocks in Outokumpu may sustain the microbial communities in this deep subsurface environment.
  • Törmä, Terhi (STKS, 2015)
    One s own Body and the Arc of Narrating The process of meaning according to Maurice Merleau-Ponty s bodily and Paul Ricoeur s narrative conception of imagination. The focus of my research is on the question of imagination in Maurice Merleau-Ponty s (1908-1961) phenomenology of the body, and Paul Ricoeur s (1913-2005) hermeneutics of narrativity. I inquire into how bodily and narrative imagination affect the process through which I make sense of myself and of my being, of others, of the world and of the communication. Through this inquiry I aim to construct a conceptual framework of religious meaning, which in its nature is multidimensional. My hypothesis is that conceptions of imagination based on the phenomenology of the body and on narrativity are mutually dependent, especially in the area of religious meaning. On a more general level, both one s own bodily wisdom and the wisdom that comes from making stories are necessary to appreciate the fullness of the meaning. In my investigation of these aspects of productive imagination I examine in detail Merleau-Ponty s early phenomenological work Phénoménologie de la perception (Phenomenology of Perception, 1945) and Ricoeur s trilogy Temps et récit (Time and Narrative, 1983-1985). My method is philosophical analysis. My dissertation comprises two main parts. Chapters three and four explore Merleau-Ponty s bodily and phenomenological conception of imagination. Because he does not express a systematic view on imagination, it is necessary to construct one on the basis of his argumentation on other subjects. This is an interesting task given the important role of imagination in his phenomenology of the body. Many aspects of imagination that are usually related to his late ontological philosophy are already present in Phénoménologie de la perception. It is through an analysis of this early work in particular that one might fully appreciate the rootedness of imagination in the body an aspect that gives some originality to Merleau-Ponty s conception compared to many others. The second main part of my research brings in the hermeneutic perspective. Chapters five and six explore Ricoeur s conception of narrative imagination, as elaborated in Temps et récit. This trilogy highlights Ricoeur s thinking on semantic innovation, on the power of language to open up the possibilities of existence. In it he presents narrative imagination as a three-part process shaping the practical field of human existence. The narrative arc he constructs extends from the prefiguration of life to the configuration of narrative (made productive by the schematic capacity of imagination), the aim being to refigure practical life through the subject who appropriates the possibilities opened up in the narrative. The capacity of narrative imagination to bring disparate elements into one plot also facilitates consideration of personal identity. In telling the story of my life I bring all the heterogeneous episodes into a meaningful unity. Ricoeur helps us to see our identity as a narrative identity: even if we cannot be the authors of our life, we may be a co-author of its significance. The idea of narrative imagination, with its focus on imaginative variation and critical distanciation, could also be applied to the religious narratives through which we refigure our everyday life and our cultural identity. The connection to practical life is an essential element in Ricoeur s conception of imagination. I show in my research how narrative imagination is based on the idea of a bodily subject. It is on this bodily level that the subject can put the effects of the narrative into practice. The aim is to free one s own possibilities and capabilities. This brings Ricoeur to the theme of l homme capable, a theme that unites his latest philosophy with the philosophical anthropology with which he began his philosophical career. Both the bodily and the narrative conceptions of imagination offer fruitful insights into religious meaning such as constituted in the everyday practices of the Evangelical Lutheran Church. For example, Merleau-Ponty s conception of one s own bodily wisdom includes the idea that understanding something is more about I can and not so much about I think, that In many instances of religious life, such as the Sunday service, it is on this level of bodily wisdom in particular that the meanings are appropriated. Kneeling with others, sensing their presence nearby, touching the bread and tasting the wine communicate the meaning of the communion on the very basic level of human existence, on the level of the body. This experience cannot become a reflected part of my life without my being able to narrate it, however. Hence, the linguistic and narrative aspects are essential in allowing my religious experiences somehow to refigure my everyday life.
  • Zhao, Xiang (Helsingin yliopisto, 2015)
    Vertebrate brain is one of the most complex and mysterious objects for biological research. Embryonic brain development involves stereotypic brain structure formation, and a vast number of precise intercellular connections are established for the generation of the highly complex circuitry of the brain. This work aims at explaining HMGB1 and AMIGO1 function in modulating vertebrate brain development. Hmgb1 knockdown zebrafish morphants produced by injection of morpholino oligonucleotides display severe defects in the forebrain and gross deteriorated catecholaminergic system. The morphant is also deficient in survival and proliferation of neural progenitors. Similar central nervous system (CNS) developmental defects have been observed in HMGB1 knockout mouse embryo. The HMGB1 null mouse embryonic brain cells showed much lower proliferating and differentiating activities compared to wild type animals. HMGB1 knockdown and knockout model respectively from zebrafish and mouse have confirmed that AMIGO1 expression is directly regulated by HMGB1. AMIGO1 regulates expression of Kv2.1 potassium channel during development, but the colocalization of AMIGO1 and Kv2.1 has only been observed in mouse and zebrafish adult brain. Furthermore, knockdown of amigo1 expression using morpholino oligonucleotides impairs the formation of fasciculated tracts in early fiber scaffolds of brain. The same defect can be also induced by mRNA-mediated expression of the Amigo1 ectodomain that inhibits adhesion mediated by the full-length protein. The impaired formation of neural circuitry is reflected in enhanced locomotor activity and attenuated escape responses. Our data demonstrate that HMGB1 is a critical factor for embryonic CNS development involved in many important developmental events. HMGB1 is essential for the neurogenesis and differentiation occurring at the developmental stage when forebrain structures are forming. Amigo1 is required for the development of neural circuits under the regulation of HMGB1. The mechanism involves homophilic interactions within the developing fiber tracts and regulation of the Kv2.1 potassium channel to form functional neural circuitry that controls locomotion. HMGB1 and AMIGO1 are both crucial for embryonic brain development and neural circuit formation.