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  • Napola, Jaakko (Helsingin yliopisto, 2014)
    Tutkimuksessa selvitettiin kuusen itä- ja keskieurooppalaisten alkuperien menestymistä ja käyttömahdollisuuksia metsänviljelyssä Etelä-Suomessa. Tutkimuksen pääaineiston muodostivat 32 kuusen alkuperäkoetta, jotka Metsäntutkimuslaitos on perustanut vuosina 1959 87, sekä viisi muuta koeviljelystä, joissa on mukana ulkomaisia alkuperiä. Pääaineiston koeviljelykset sisältävät 149 ulkomaista alkuperää 16 maantieteelliseltä lähtöisyysalueelta. Alkuperät ryhmiteltiin alankoaineistoon (Baltia, Länsi-Venäjä, Valko-Venäjä ja Koillis-Puola) sekä vuoristoaineistoon (muiden muassa Böhmerwald, Sudeetit-Beskidit, Tatra ja Karpaatit). Kokeiden ikä oli mitattaessa 15 40 kasvukautta (keskimäärin 25 kasvukautta) istutuksesta. Eri koeviljelyksistä saadut tulokset muutettiin yhteismitallisiksi suhteuttamalla ne suomalaisten vertailuerien keskiarvoon. Tarkasteltuja kasvu- ja laatutunnuksia olivat pituus, rinnankorkeusläpimitta, rungon keskitilavuus ja puuston tilavuus sekä elävyys, poikaoksat ja korot. Korolla tarkoitetaan rungon kuoren halkeamaa tai muuta vaurioita, jossa on mustaa sienikasvustoa. Siemenen siirtomatkan eli leveys- ja pituusastesiirron sekä koepaikan lämpösumman vaikutusta elävyyteen, kasvuun ja rungon laatuun tutkittiin alankoaineistossa lineaarisen sekamallin avulla. Selitysasteet jäivät alhaisiksi. Korkein selitysaste oli poikaoksapuiden osuutta selittävällä mallilla, 38,7 prosenttia. Alankoaineistossa suhteellinen elävyys oli keskimäärin 97 prosenttia suomalaisten vertailuerien keskiarvosta (= 100) ja suhteellinen runkotilavuus 113 prosenttia. Koropuiden suhteellinen osuus oli alankoaineistossa 103 prosenttia ja poikaoksapuiden suhteellinen osuus 90 prosenttia. Käyttökelpoisin lähtöisyysalue metsänviljelyn kannalta on Viro. Virolaisia kuusialkuperiä voidaan viljellä Etelä-Suomessa alueilla, joiden lämpösumma on yli 1250 d.d. Länsi-Venäjän alkuperiä (Pihkova ja Novgorod) voidaan toistaiseksi suositella käytettäväksi vain etelärannikolla, koska sisämaassa ei ole näillä alkuperillä perustettuja koeviljelyksiä. Latvialaiset alkuperät menestyvät hyvin etelärannikolla ja paikoin sisämaassa, mutta niiden kasvatukseen liittyy kohonnut korovaurioiden riski. Valko-Venäjän ja Koillis-Puolan alkuperät ovat nopeakasvuisia, mutta korojen yleisyyden vuoksi niitä ei voida suositella käytettäväksi Suomessa. Keski-Euroopan vuoristoalkuperistä parhaiten menestyvät Böhmerwaldin, Tatran ja Karpaattien alkuperät. Näitäkään alkuperiä ei kuitenkaan voida suositella käytettäväksi Suomessa niiden kasvatukseen liittyvien riskien takia. Tutkimus vahvistaa oikeaksi nykyisen suosituksen, jonka mukaan Viron alkuperiä voidaan viljellä Suomessa Salpausselän eteläpuolella.
  • Harmanen, Heikki (2007)
  • Uimonen, Sirpa (Helsingin yliopisto, 2011)
    The purpose of this study was to find out whether food-related lifestyle guides and explains product evaluations, specifically, consumer perceptions and choice evaluations of five different food product categories: lettuce, mincemeat, savoury sauce, goat cheese, and pudding. The opinions of consumers who shop in neighbourhood stores were considered most valuable. This study applies means-end chain (MEC) theory, according to which products are seen as means by which consumers attain meaningful goals. The food-related lifestyle (FRL) instrument was created to study lifestyles that reflect these goals. Further, this research has adopted the view that the FRL functions as a script which guides consumer behaviour. Two research methods were used in this study. The first was the laddering interview, the primary aim of which was to gather information for formulating the questionnaire of the main study. The survey consisted of two separate questionnaires. The first was the FRL questionnaire modified for this study. The aim of the other questionnaire was to determine the choice criteria for buying five different categories of food products. Before these analyses could be made, several data modifications were made following MEC analysis procedures. Beside forming FRL dimensions by counting sum-scores from the FRL statements, factor analysis was run in order to elicit latent factors underlying the dimensions. The lifestyle factors found were adventurous, conscientious, enthusiastic, snacking, moderate, and uninvolved lifestyles. The association analyses were done separately for each choice of product as well as for each attribute-consequence linkage with a non-parametric Mann-Whitney U test. The testing variables were FRL dimensions and the FRL lifestyle factors. In addition, the relation between the attribute-consequence linkages and the demographic variables were analysed. Results from this study showed that the choice of product is sequential, so that consumers first categorize products into groups based on specific criteria like health or convenience. It was attested that the food-related lifestyles function as a script in food choice and that the FRL instrument can be used to predict consumer buying behaviour. Certain lifestyles were associated with the choice of each product category. The actual product choice within a product category then appeared to be a different matter. In addition, this study proposes a modification to the FRL instrument. The positive towards advertising FRL dimension was modified to examine many kinds of information search including the internet, TV, magazines, and other people. This new dimension, which was designated as being open to additional information, proved to be very robust and reliable in finding differences in consumer choice behaviour. Active additional information search was linked to adventurous and snacking food-related lifestyles. The results of this study support the previous knowledge that consumers expect to get many benefits simultaneously when they buy food products. This study brought detailed information about the benefits sought, the combination of benefits differing between products and between respondents. Household economy, pleasure and quality were emphasized with the choice of lettuce. Quality was the most significant benefit in choosing mincemeat, but health related benefits were often evaluated as well. The dominant benefits linked to savoury sauce were household economic benefits, expected pleasurable experiences, and a lift in self-respect. The choice of goat cheese appeared not to be an economic decision, self-respect, pleasure, and quality being included in the choice criteria. In choosing pudding, the respondents considered the well-being of family members, and indulged their family members or themselves.
  • Heikkilä, Mari (Helsingin yliopisto, 2007)
    The chemical composition of breast milk has been studied in detail in the past decades. Hundreds of new antibacterial and antiviral components have been found. Several molecules have been found to promote the proper function of neonatal intestine. However, microbiological studies of breast milk have been, until recently, focused mainly on detecting harmful and pathogenic bacteria and viruses. Natural microbial diversity of human milk has not been widely studied before the work reported in this thesis. This is mainly because breast milk has traditionally been thought to be sterile - even if a certain amount of commensal bacteria have usually been detected in milk samples. The first part of this licentiate thesis contains a short literature review about the anatomy and physiology of breast feeding, human milk chemical and microbiological composition, mastitis, intestinal flora and bacteriocins. The second part reports on the experiments of the licentiate work, concentrating on the microbial diversity in the milk of healthy breast-feeding mothers, and the ability of these bacteria to produce antibacterial substances against pathogenic bacteria. The results indicate that human milk is a source of commensal bacteria for infant intestine. 509 random isolates from 40 breast milk samples were isolated and identified by 16S rRNA sequencing. Median bacterial count was about 600 colony forming units per milliliter. Over half of the isolates were staphylococci, and almost one third streptococci. The most common species were skin bacteria Staphylococcus epidermidis and oral bacteria Streptococcus salivarius and Streptococcus mitis. Lactic acid bacteria, identified as members of Lactobacillus-, Lactococcus- and Leuconostoc -genera, were found in five milk samples. Enterococci were found in three samples. A novel finding in this study is the capability of these commensal bacteria to inhibit the growth of pathogens. In 90 precent of the milk samples commensal bacteria inhibiting the growth of Staphylococcus aureus were found. In 40 precent of samples the colonies could block the growth completely. One fifth of the isolated Staph. epidermidis strains, half of Str. salivarius strains, and all lactic acid bacteria and enterococci could inhibit or block the growth of Staph. aureus. In further study also Listeria innocua- and Micrococcus luteus active isolates were found in 33 and 11 precent of milk samples (out of 140). Furthermore, two Lactococcus lactis isolates from the breast milk were shown to produce bacteriocin nisin, which is an antimicrobial molecule used as a food preservative. The importance of these human milk commensal bacteria in the development of newborn intestinal flora and immune system, as well as in preventing maternal breast infections, should be further explored.