Study of the small heat shock proteins in the normal and wooden breast chicken muscles

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Title: Study of the small heat shock proteins in the normal and wooden breast chicken muscles
Author: Shanthi Venkatesan, Aadhavan
Contributor: University of Helsinki, Faculty of Agriculture and Forestry, Department of Food and Environmental Sciences
Publisher: Helsingfors universitet
Date: 2017
Language: eng
Thesis level: master's thesis
Discipline: Bioteknik (EYT)
Biotechnology (EYT)
Biotekniikka (EYT)
Abstract: A new myopathy called “wooden breast” affecting Pectoralis major muscles of broilers was described by researchers in Finland and other countries and it has affected the economy of the poultry industry in the last few years. The exact mechanisms behind this myopathy are yet to be discovered. This study focused on establishing any relationship between this new myodegeneration disorder “wooden breast” and small heat shock proteins. The primary aim of this work was to study the 3 important small heat shock proteins such as HSP20, HSP27 and αβ-crystallin in wooden breast and normal breast fillets by means of immunoblot technique. Six random fillet samples identified as wooden breast and six normal breast muscle samples were selected for this study. The presence of small heat shock protein HSP27 was comparatively higher in wooden breast muscles in both the myofibrillar fraction (p-value = 0. 02) and sarcoplasmic muscle fraction (p-value = 0. 01) than their presence in the normal breast muscles. Stress suffered by the birds belonging to the wooden breast condition is suggested to upregulate the heat shock proteins to antagonize against protein misfolding, protein denaturation and for protection of myofibrillar and structural proteins. As an outcome of the study, a proposal can be made about the possible mechanism behind the wooden breast to be ischemia and heat stress causing the upregulation of heat shock proteins, which was supported by the presence of HSP27 in both the myofibrillar and the sarcoplasmic muscle fraction. The presence of a relative higher amount of HSP27 in wooden breast muscles suggests that they defend against the stress suffered by the bird. Detecting the exact time point of onset of stress and factors causing the stress might unravel more information about this disorder, and studying the presence of small heat shock proteins at different time points post slaughter till the start of rigor mortis might provide additional details behind this muscle disorder.
Subject: Chaperones
myofibrillar proteins
sarcoplasmic proteins
small heat shock proteins

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