Gene Deletion in Barley Mediated by LTR-retrotransposon BARE

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http://hdl.handle.net/10138/181508

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Shang , Y , Yang , F , Schulman , A H , Zhu , J , Jia , Y , Wang , J , Zhang , X-Q , Jia , Q , Hua , W , Yang , J & Li , C 2017 , ' Gene Deletion in Barley Mediated by LTR-retrotransposon BARE ' , Scientific Reports , vol. 7 , 43766 . https://doi.org/10.1038/srep43766

Title: Gene Deletion in Barley Mediated by LTR-retrotransposon BARE
Author: Shang, Yi; Yang, Fei; Schulman, Alan H.; Zhu, Jinghuan; Jia, Yong; Wang, Junmei; Zhang, Xiao-Qi; Jia, Qiaojun; Hua, Wei; Yang, Jianming; Li, Chengdao
Contributor: University of Helsinki, University of Helsinki
Date: 2017-03-02
Language: eng
Number of pages: 9
Belongs to series: Scientific Reports
ISSN: 2045-2322
URI: http://hdl.handle.net/10138/181508
Abstract: A poly-row branched spike (prbs) barley mutant was obtained from soaking a two-rowed barley inflorescence in a solution of maize genomic DNA. Positional cloning and sequencing demonstrated that the prbs mutant resulted from a 28 kb deletion including the inflorescence architecture gene HvRA2. Sequence annotation revealed that the HvRA2 gene is flanked by two LTR (long terminal repeat) retrotransposons (BARE) sharing 89% sequence identity. A recombination between the integrase (IN) gene regions of the two BARE copies resulted in the formation of an intact BARE and loss of HvRA2. No maize DNA was detected in the recombination region although the flanking sequences of HvRA2 gene showed over 73% of sequence identity with repetitive sequences on 10 maize chromosomes. It is still unknown whether the interaction of retrotransposons between barley and maize has resulted in the recombination observed in the present study.
Subject: MULTIPLE HORIZONTAL TRANSFERS
DOUBLE-STRANDED DNA
TRANSPOSABLE ELEMENTS
MAIZE GENOME
EVOLUTION
HORDEUM
PLANTS
RECOMBINATION
ACCUMULATION
MORPHOLOGY
1184 Genetics, developmental biology, physiology
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