Genomic and phenomic screens for flower related RING type ubiquitin E3 ligases in Arabidopsis

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Pavicic , M , Mouhu , K , Wang , F , Bilicka , M M , Chovanček , E & Himanen , K I H 2017 , ' Genomic and phenomic screens for flower related RING type ubiquitin E3 ligases in Arabidopsis ' , Frontiers in plant science , vol. 8 , 416 . https://doi.org/10.3389/fpls.2017.00416

Title: Genomic and phenomic screens for flower related RING type ubiquitin E3 ligases in Arabidopsis
Author: Pavicic, Mirko; Mouhu, Katriina; Wang, Feng; Bilicka, Marcelina Marta; Chovanček, Erik; Himanen, Kristiina Irma Helena
Contributor: University of Helsinki, Department of Agricultural Sciences
University of Helsinki, Department of Agricultural Sciences
University of Helsinki, Department of Agricultural Sciences
University of Helsinki, Plant Phenomics
University of Helsinki, Biosciences
Date: 2017-03-28
Language: eng
Number of pages: 17
Belongs to series: Frontiers in plant science
ISSN: 1664-462X
URI: http://hdl.handle.net/10138/181663
Abstract: Flowering time control integrates endogenous as well as environmental signals to promote flower development. The pathways and molecular networks involved are complex and integrate many modes of signal transduction. In plants ubiquitin mediated protein degradation pathway has been proposed to be as important mode of signaling as phosphorylation and transcription. To systematically study the role of ubiquitin signaling in the molecular regulation of flowering we have taken a genomic approach to identify flower related Ubiquitin Proteasome System components. As a large and versatile gene family the RING type ubiquitin E3 ligases were chosen as targets of the genomic screen. To this end the complete list of Arabidopsis RING E3 ligases were retrieved and verified in the Arabidopsis genome v11. Their differential expression was used for their categorization into flower organs or developmental stages. Known regulators of flowering time or floral organ development were identified in these categories through literature search and representative mutants for each category were purchased for functional characterization by growth and morphological phenotyping. To this end, a workflow was developed for high throughput phenotypic screening of growth, morphology and flowering of nearly a thousand Arabidopsis plants in one experimental round.
Subject: 414 Agricultural biotechnology
Arabidopsis
flower
RING E3 ligase
ubiquitin
high throughput
image based phenotyping
phenomics data analysis
ZINC-FINGER PROTEIN
PROTEASOME SYSTEM
GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE
CONSTANS EXPRESSION
GENE-EXPRESSION
GABI-KAT
THALIANA
plant
time
information
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