Development of a high-throughput colorimetric Zika virus infection assay

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http://hdl.handle.net/10138/182236

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Mueller , J A , Harms , M , Schubert , A , Mayer , B , Jansen , S , Herbeuval , J-P , Michel , D , Mertens , T , Vapalahti , O , Schmidt-Chanasit , J & Muench , J 2017 , ' Development of a high-throughput colorimetric Zika virus infection assay ' , Medical Microbiology and Immunology , vol. 206 , no. 2 , pp. 175-185 . https://doi.org/10.1007/s00430-017-0493-2

Title: Development of a high-throughput colorimetric Zika virus infection assay
Author: Mueller, Janis A.; Harms, Mirja; Schubert, Axel; Mayer, Benjamin; Jansen, Stephanie; Herbeuval, Jean-Philippe; Michel, Detlef; Mertens, Thomas; Vapalahti, Olli; Schmidt-Chanasit, Jonas; Muench, Jan
Contributor: University of Helsinki, Medicum
Date: 2017-04
Language: eng
Number of pages: 11
Belongs to series: Medical Microbiology and Immunology
ISSN: 0300-8584
URI: http://hdl.handle.net/10138/182236
Abstract: Zika virus (ZIKV) is an emerging pathogen that causes congenital infections which may result in birth defects, such as microcephaly. Currently, no approved treatment or vaccination is available. ZIKV can be readily detected in cell culture where virally infected cells are normally stained by specific antibodies. As ZIKV regularly causes a cytopathic effect, we were wondering whether this viral property can be used to quantitatively determine viral infectivity. We here describe the use of an 3-[4,5-dimethyl-2-thiazolyl]-2,5-diphenyl-2H-tetrazolium bromide-(MTT)-based cell viability assay that allows to determine ZIKV-induced cell death. We show that this colorimetric assay quantifies ZIKV infection over a broad range of viral dilutions in both monkey and human cells. It allows to determine inhibitory activities of antivirals that block ZIKV or to define the neutralizing antibody titers of ZIKV antisera. This MTT-based ZIKV detection assay can be evaluated by naked eye or computational tools, has a broad linear range, does not require large equipment or costly reagents, and thus represents a promising alternative to antibody-based assays, in particular in resource-poor settings. We propose to use this simple, fast, and cheap method for quantification of ZIKV neutralizing antibodies and testing of antiviral compounds.
Subject: MTT
ZIKV
Zika virus
PRNT
Screening
HERPES-SIMPLEX-VIRUS
ANTI-HIV COMPOUNDS
ANTIBODIES
INACTIVATION
REPLICATION
MICRONESIA
1183 Plant biology, microbiology, virology
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