beta carbonic anhydrase is required for female fertility in Drosophila melanogaster

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Syrjänen , L , Valanne , S , Kuuslahti , M , Tuomela , T , Sriram , A , Sanz , A , Jacobs , H T , Rämet , M & Parkkila , S 2015 , ' beta carbonic anhydrase is required for female fertility in Drosophila melanogaster ' Frontiers in Zoology , vol. 12 , 19 . DOI: 10.1186/s12983-015-0111-3

Title: beta carbonic anhydrase is required for female fertility in Drosophila melanogaster
Author: Syrjänen, Leo; Valanne, Susanna; Kuuslahti, Marianne; Tuomela, Tea; Sriram, Ashwin; Sanz, Alberto; Jacobs, Howard T.; Rämet, Mika; Parkkila, Seppo
Contributor: University of Helsinki, Institute of Biotechnology
Date: 2015-08-22
Language: eng
Number of pages: 10
Belongs to series: Frontiers in Zoology
ISSN: 1742-9994
URI: http://hdl.handle.net/10138/182603
Abstract: Background: Carbonic anhydrases (CAs, EC 4.2.1.1) are ubiquitous enzymes that catalyze the reversible hydration reaction of carbon dioxide. CAs are present as six structurally divergent enzyme families: alpha, beta, gamma, delta, zeta and eta. beta-CAs have a wide distribution across different species including invertebrates. Previously, we showed that Drosophila melanogaster beta-CA is a highly active mitochondrial enzyme. In this study, we investigated the function of Drosophila beta-CA by silencing the expression of the beta-CA gene using UAS/GAL4-based RNA interference (RNAi) in Drosophila in vivo. Results: Crossing beta-CA RNAi lines over ubiquitous Actin driver flies did not produce any viable progeny, indicating that beta-CA expression is required for fly development. RNAi silencing of beta-CA ubiquitously in adult flies did not affect their survival rate or function of mitochondrial electron transport chain. Importantly, beta-CA RNAi led to impaired reproduction. All beta-CA knockdown females were sterile, and produced few or no eggs. Whole ovaries of knockdown females looked normal but upon cadherin staining, there was an apparent functional defect in migration of border cells, which are considered essential for normal fertilization. Conclusions: These results indicate that although Drosophila beta-CA is dispensable for survival of adult flies, it is essential for female fertility.
Subject: ALTERNATIVE OXIDASE
CELL-MIGRATION
BORDER CELLS
EXPRESSION
PHENOTYPES
OOGENESIS
DISEASE
MODEL
PH
1181 Ecology, evolutionary biology
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