A novel consortium of Lactobacillus rhamnosus and Streptococcus thermophilus for increased access to functional fermented foods

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Kort , R , Westerik , N , Serrano , L M , Douillard , F P , Gottstein , W , Mukisa , I M , Tuijn , C J , Basten , L , Hafkamp , B , Meijer , W C , Teusink , B , de Vos , W M , Reid , G & Sybesma , W 2015 , ' A novel consortium of Lactobacillus rhamnosus and Streptococcus thermophilus for increased access to functional fermented foods ' , Microbial Cell Factories , vol. 14 , 195 . https://doi.org/10.1186/s12934-015-0370-x

Title: A novel consortium of Lactobacillus rhamnosus and Streptococcus thermophilus for increased access to functional fermented foods
Author: Kort, Remco; Westerik, Nieke; Serrano, L. Mariela; Douillard, Francois P.; Gottstein, Willi; Mukisa, Ivan M.; Tuijn, Coosje J.; Basten, Lisa; Hafkamp, Bert; Meijer, Wilco C.; Teusink, Bas; de Vos, Willem M.; Reid, Gregor; Sybesma, Wilbert
Contributor organization: Departments of Faculty of Veterinary Medicine
Veterinary Biosciences
Veterinary Microbiology and Epidemiology
Willem Meindert Vos de / Principal Investigator
Immunobiology Research Program
Research Programs Unit
Department of Bacteriology and Immunology
de Vos & Salonen group
Date: 2015-12-08
Language: eng
Number of pages: 13
Belongs to series: Microbial Cell Factories
ISSN: 1475-2859
DOI: https://doi.org/10.1186/s12934-015-0370-x
URI: http://hdl.handle.net/10138/182615
Abstract: Background: The lactic acid bacterium Lactobacillus rhamnosus GG is the most studied probiotic bacterium with proven health benefits upon oral intake, including the alleviation of diarrhea. The mission of the Yoba for Life foundation is to provide impoverished communities in Africa increased access to Lactobacillus rhamnosus GG under the name Lactobacillus rhamnosus yoba 2012, world's first generic probiotic strain. We have been able to overcome the strain's limitations to grow in food matrices like milk, by formulating a dried starter consortium with Streptococcus thermophilus that enables the propagation of both strains in milk and other food matrices. The affordable seed culture is used by people in resource-poor communities. Results: We used S. thermophilus C106 as an adjuvant culture for the propagation of L. rhamnosus yoba 2012 in a variety of fermented foods up to concentrations, because of its endogenous proteolytic activity, ability to degrade lactose and other synergistic effects. Subsequently, L. rhamnosus could reach final titers of 1E+09 CFU ml(-1), which is sufficient to comply with the recommended daily dose for probiotics. The specific metabolic interactions between the two strains were derived from the full genome sequences of L. rhamnosus GG and S. thermophilus C106. The piliation of the L. rhamnosus yoba 2012, required for epithelial adhesion and inflammatory signaling in the human host, was stable during growth in milk for two rounds of fermentation. Sachets prepared with the two strains, yoba 2012 and C106, retained viability for at least 2 years. Conclusions: A stable dried seed culture has been developed which facilitates local and low-cost production of a wide range of fermented foods that subsequently act as delivery vehicles for beneficial bacteria to communities in east Africa.
Subject: Enrichment
Lactobacillus rhamnosus GG
Lactobacillus rhamnosus yoba 2012
Streptococcus thermophilus C106
Bacterial fermentation
Functional foods
Fermented foods
3111 Biomedicine
416 Food Science
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion

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