Role for formin-like 1-dependent acto-myosin assembly in lipid droplet dynamics and lipid storage

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Pfisterer , S , Gateva , G , Horvath , P , Pirhonen , J , Salo , V T , Karhinen , L , Varjosalo , M , Ryhänen , S J , Lappalainen , P & Ikonen , E 2017 , ' Role for formin-like 1-dependent acto-myosin assembly in lipid droplet dynamics and lipid storage ' , Nature Communications , vol. 8 , 14858 . https://doi.org/10.1038/ncomms14858

Title: Role for formin-like 1-dependent acto-myosin assembly in lipid droplet dynamics and lipid storage
Author: Pfisterer, Simon; Gateva, Gergana; Horvath, Peter; Pirhonen, Juho; Salo, Veijo T.; Karhinen, Leena; Varjosalo, Markku; Ryhänen, Samppa J.; Lappalainen, Pekka; Ikonen, Elina
Other contributor: University of Helsinki, Medicum
University of Helsinki, Institute of Biotechnology
University of Helsinki, Institute for Molecular Medicine Finland
University of Helsinki, Medicum
University of Helsinki, Medicum
University of Helsinki, Medicum
University of Helsinki, Institute of Biotechnology
University of Helsinki, Children's Hospital
University of Helsinki, Institute of Biotechnology
University of Helsinki, Medicum












Date: 2017-03-31
Language: eng
Number of pages: 14
Belongs to series: Nature Communications
ISSN: 2041-1723
DOI: https://doi.org/10.1038/ncomms14858
URI: http://hdl.handle.net/10138/183452
Abstract: Lipid droplets (LDs) are cellular organelles specialized in triacylglycerol (TG) storage undergoing homotypic clustering and fusion. In non-adipocytic cells with numerous LDs this is balanced by poorly understood droplet dissociation mechanisms. We identify non-muscle myosin IIa (NMIIa/MYH-9) and formin-like 1 (FMNL1) in the LD proteome. NMIIa and actin filaments concentrate around LDs, and form transient foci between dissociating LDs. NMIIa depletion results in decreased LD dissociations, enlarged LDs, decreased hydrolysis and increased storage of TGs. FMNL1 is required for actin assembly on LDs in vitro and for NMIIa recruitment to LDs in cells. We propose a novel acto-myosin structure regulating lipid storage: FMNL1-dependent assembly of myosin II-functionalized actin filaments on LDs facilitates their dissociation, thereby affecting LD surface-to-volume ratio and enzyme accessibility to TGs. In neutrophilic leucocytes from MYH9-related disease patients NMIIa inclusions are accompanied by increased lipid storage in droplets, suggesting that NMIIa dysfunction may contribute to lipid imbalance in man.
Subject: RAMAN SCATTERING MICROSCOPY
MAMMALIAN-CELLS
PROTEINS
CYTOKINESIS
RECRUITMENT
INHIBITOR
FUSION
GROWTH
1182 Biochemistry, cell and molecular biology
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