Pfisterer , S , Gateva , G , Horvath , P , Pirhonen , J , Salo , V T , Karhinen , L , Varjosalo , M , Ryhänen , S J , Lappalainen , P & Ikonen , E 2017 , ' Role for formin-like 1-dependent acto-myosin assembly in lipid droplet dynamics and lipid storage ' , Nature Communications , vol. 8 , 14858 . https://doi.org/10.1038/ncomms14858
Title: | Role for formin-like 1-dependent acto-myosin assembly in lipid droplet dynamics and lipid storage |
Author: | Pfisterer, Simon; Gateva, Gergana; Horvath, Peter; Pirhonen, Juho; Salo, Veijo T.; Karhinen, Leena; Varjosalo, Markku; Ryhänen, Samppa J.; Lappalainen, Pekka; Ikonen, Elina |
Contributor organization: | Medicum Department of Anatomy Research Programs Unit Institute of Biotechnology Institute for Molecular Medicine Finland Molecular Systems Biology Children's Hospital Clinicum Hematologian yksikkö Department of Medicine University of Helsinki Pekka Lappalainen / Principal Investigator HUS Children and Adolescents |
Date: | 2017-03-31 |
Language: | eng |
Number of pages: | 14 |
Belongs to series: | Nature Communications |
ISSN: | 2041-1723 |
DOI: | https://doi.org/10.1038/ncomms14858 |
URI: | http://hdl.handle.net/10138/183452 |
Abstract: | Lipid droplets (LDs) are cellular organelles specialized in triacylglycerol (TG) storage undergoing homotypic clustering and fusion. In non-adipocytic cells with numerous LDs this is balanced by poorly understood droplet dissociation mechanisms. We identify non-muscle myosin IIa (NMIIa/MYH-9) and formin-like 1 (FMNL1) in the LD proteome. NMIIa and actin filaments concentrate around LDs, and form transient foci between dissociating LDs. NMIIa depletion results in decreased LD dissociations, enlarged LDs, decreased hydrolysis and increased storage of TGs. FMNL1 is required for actin assembly on LDs in vitro and for NMIIa recruitment to LDs in cells. We propose a novel acto-myosin structure regulating lipid storage: FMNL1-dependent assembly of myosin II-functionalized actin filaments on LDs facilitates their dissociation, thereby affecting LD surface-to-volume ratio and enzyme accessibility to TGs. In neutrophilic leucocytes from MYH9-related disease patients NMIIa inclusions are accompanied by increased lipid storage in droplets, suggesting that NMIIa dysfunction may contribute to lipid imbalance in man. |
Subject: |
RAMAN SCATTERING MICROSCOPY
MAMMALIAN-CELLS PROTEINS CYTOKINESIS RECRUITMENT INHIBITOR FUSION GROWTH 1182 Biochemistry, cell and molecular biology |
Peer reviewed: | Yes |
Rights: | unspecified |
Usage restriction: | openAccess |
Self-archived version: | publishedVersion |
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