Regulatory Architecture of Gene Expression Variation in the Threespine Stickleback Gasterosteus aculeatus

Show full item record



Permalink

http://hdl.handle.net/10138/183463

Citation

Pritchard , V L , Viitaniemi , H M , McCairns , R J S , Merila , J , Nikinmaa , M , Primmer , C R & Leder , E H 2017 , ' Regulatory Architecture of Gene Expression Variation in the Threespine Stickleback Gasterosteus aculeatus ' , G3 - Genes genomes genetics , vol. 7 , no. 1 , pp. 165-178 . https://doi.org/10.1534/g3.116.033241

Title: Regulatory Architecture of Gene Expression Variation in the Threespine Stickleback Gasterosteus aculeatus
Author: Pritchard, Victoria L.; Viitaniemi, Heidi M.; McCairns, R. J. Scott; Merila, Juha; Nikinmaa, Mikko; Primmer, Craig R.; Leder, Erica H.
Contributor: University of Helsinki, University of Helsinki
University of Helsinki, Biosciences
University of Helsinki, Univ Turku, Turku Ctr Biotechnol
Date: 2017-01
Language: eng
Number of pages: 14
Belongs to series: G3 - Genes genomes genetics
ISSN: 2160-1836
URI: http://hdl.handle.net/10138/183463
Abstract: Much adaptive evolutionary change is underlain by mutational variation in regions of the genome that regulate gene expression rather than in the coding regions of the genes themselves. An understanding of the role of gene expression variation in facilitating local adaptation will be aided by an understanding of underlying regulatory networks. Here, we characterize the genetic architecture of gene expression variation in the threespine stickleback (Gasterosteus aculeatus), an important model in the study of adaptive evolution. We collected transcriptomic and genomic data from 60 half-sib families using an expression microarray and genotyping-by-sequencing, and located expression quantitative trait loci (eQTL) underlying the variation in gene expression in liver tissue using an interval mapping approach. We identified eQTL for several thousand expression traits. Expression was influenced by polymorphism in both cis- and trans-regulatory regions. Transe-QTL clustered into hotspots. We did not identify master transcriptional regulators in hotspot locations: rather, the presence of hotspots may be driven by complex interactions between multiple transcription factors. One observed hotspot colocated with a QTL recently found to underlie salinity tolerance in the threespine stickleback. However, most other observed hotspots did not colocate with regions of the genome known to be involved in adaptive divergence between marine and freshwater habitats.
Subject: Baltic Sea
eQTL
gene expression
liver
threespine stickleback
QUANTITATIVE TRAIT LOCI
EVOLVED TOOTH GAIN
FRESH-WATER
3-SPINED STICKLEBACK
ADAPTIVE DIVERGENCE
ANALYSIS REVEALS
LAKE WHITEFISH
EVOLUTION
SELECTION
HYPOXIA
1181 Ecology, evolutionary biology
Rights:


Files in this item

Total number of downloads: Loading...

Files Size Format View
165.full.pdf 1.265Mb PDF View/Open

This item appears in the following Collection(s)

Show full item record