Nanoscale Membrane Domain Formation Driven by Cholesterol

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dc.contributor.author Javanainen, Matti
dc.contributor.author Martinez-Seara, Hector
dc.contributor.author Vattulainen, Ilpo
dc.date.accessioned 2017-05-18T07:09:02Z
dc.date.available 2017-05-18T07:09:02Z
dc.date.issued 2017-04-25
dc.identifier.citation Javanainen , M , Martinez-Seara , H & Vattulainen , I 2017 , ' Nanoscale Membrane Domain Formation Driven by Cholesterol ' , Scientific Reports , vol. 7 , 1143 . https://doi.org/10.1038/s41598-017-01247-9
dc.identifier.other PURE: 84373162
dc.identifier.other PURE UUID: 24c42665-1323-4bd7-9cd3-7b1d803c1a41
dc.identifier.other WOS: 000400104200032
dc.identifier.other Scopus: 85018985901
dc.identifier.other ORCID: /0000-0001-7408-3214/work/32975996
dc.identifier.other ORCID: /0000-0003-4858-364X/work/38784215
dc.identifier.uri http://hdl.handle.net/10138/185239
dc.description.abstract Biological membranes generate specific functions through compartmentalized regions such as cholesterol-enriched membrane nanodomains that host selected proteins. Despite the biological significance of nanodomains, details on their structure remain elusive. They cannot be observed via microscopic experimental techniques due to their small size, yet there is also a lack of atomistic simulation models able to describe spontaneous nanodomain formation in sufficiently simple but biologically relevant complex membranes. Here we use atomistic simulations to consider a binary mixture of saturated dipalmitoylphosphatidylcholine and cholesterol - the "minimal standard" for nanodomain formation. The simulations reveal how cholesterol drives the formation of fluid cholesterol-rich nanodomains hosting hexagonally packed cholesterol-poor lipid nanoclusters, both of which show registration between the membrane leaflets. The complex nanodomain substructure forms when cholesterol positions itself in the domain boundary region. Here cholesterol can also readily flip-flop across the membrane. Most importantly, replacing cholesterol with a sterol characterized by a less asymmetric ring region impairs the emergence of nanodomains. The model considered explains a plethora of controversial experimental results and provides an excellent basis for further computational studies on nanodomains. Furthermore, the results highlight the role of cholesterol as a key player in the modulation of nanodomains for membrane protein function. en
dc.format.extent 10
dc.language.iso eng
dc.relation.ispartof Scientific Reports
dc.rights cc_by
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject MOLECULAR-DYNAMICS SIMULATIONS
dc.subject DIFFERENTIAL SCANNING CALORIMETRY
dc.subject PULMONARY SURFACTANT MEMBRANES
dc.subject LIPID-BILAYERS
dc.subject PHASE-BEHAVIOR
dc.subject PHOSPHATIDYLCHOLINE BILAYERS
dc.subject MAGNETIC-RESONANCE
dc.subject MIXTURES
dc.subject ORDER
dc.subject RAFTS
dc.subject 114 Physical sciences
dc.subject 1182 Biochemistry, cell and molecular biology
dc.title Nanoscale Membrane Domain Formation Driven by Cholesterol en
dc.type Article
dc.contributor.organization Department of Physics
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.1038/s41598-017-01247-9
dc.relation.issn 2045-2322
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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