In muro deacetylation of xylan affects lignin properties and improves saccharification of aspen wood

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Pawar , P M-A , Derba-Maceluch , M , Chong , S-L , Gandla , M L , Bashar , S S , Sparrman , T , Ahvenainen , P , Hedenström , M , Ozparpucu , M , Ruggeberg , M , Serimaa , R , Lawoko , M , Tenkanen , M , Jönsson , L J & Mellerowicz , E J 2017 , ' In muro deacetylation of xylan affects lignin properties and improves saccharification of aspen wood ' , Biotechnology for Biofuels , vol. 10 , 98 . https://doi.org/10.1186/s13068-017-0782-4

Title: In muro deacetylation of xylan affects lignin properties and improves saccharification of aspen wood
Author: Pawar, Prashant Mohan-Anupama; Derba-Maceluch, Marta; Chong, Sun-Li; Gandla, Madhavi Latha; Bashar, Shamrat Shafiul; Sparrman, Tobias; Ahvenainen, Patrik; Hedenström, Mattias; Ozparpucu, Merve; Ruggeberg, Markus; Serimaa, Ritva; Lawoko, Martin; Tenkanen, Maija; Jönsson, Leif J.; Mellerowicz, Ewa J.
Contributor: University of Helsinki, Chalmers, Chalmers University of Technology
University of Helsinki, Department of Physics
University of Helsinki, Department of Physics
University of Helsinki, Department of Food and Nutrition
Date: 2017-04-20
Language: eng
Number of pages: 11
Belongs to series: Biotechnology for Biofuels
ISSN: 1754-6834
URI: http://hdl.handle.net/10138/185240
Abstract: Background: Lignocellulose from fast growing hardwood species is a preferred source of polysaccharides for advanced biofuels and "green" chemicals. However, the extensive acetylation of hardwood xylan hinders lignocellulose saccharification by obstructing enzymatic xylan hydrolysis and causing inhibitory acetic acid concentrations during microbial sugar fermentation. To optimize lignocellulose for cost-effective saccharification and biofuel production, an acetyl xylan esterase AnAXE1 from Aspergillus niger was introduced into aspen and targeted to cell walls. Results: AnAXE1-expressing plants exhibited reduced xylan acetylation and grew normally. Without pretreatment, their lignocellulose yielded over 25% more glucose per unit mass of wood (dry weight) than wild-type plants. Glucose yields were less improved (+7%) after acid pretreatment, which hydrolyses xylan. The results indicate that AnAXE1 expression also reduced the molecular weight of xylan, and xylan-lignin complexes and/or lignin co-extracted with xylan, increased cellulose crystallinity, altered the lignin composition, reducing its syringyl to guaiacyl ratio, and increased lignin solubility in dioxane and hot water. Lignin-associated carbohydrates became enriched in xylose residues, indicating a higher content of xylo-oligosaccharides. Conclusions: This work revealed several changes in plant cell walls caused by deacetylation of xylan. We propose that deacetylated xylan is partially hydrolyzed in the cell walls, liberating xylo-oligosaccharides and their associated lignin oligomers from the cell wall network. Deacetylating xylan thus not only increases its susceptibility to hydrolytic enzymes during saccharification but also changes the cell wall architecture, increasing the extractability of lignin and xylan and facilitating saccharification.
Subject: Acetylation
Xylan
Saccharification
Wood
Populus
PLANT-CELL WALL
O-ACETYLATION
SECONDARY WALL
ARABIDOPSIS-THALIANA
ENZYMATIC-HYDROLYSIS
NMR-SPECTROSCOPY
DISTINCT ROLES
BIOSYNTHESIS
ESTERASE
RELEASE
1183 Plant biology, microbiology, virology
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