Significant Role of Collagen XVII And Integrin beta 4 in Migration and Invasion of The Less Aggressive Squamous Cell Carcinoma Cells

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Moilanen , J M , Loeffek , S , Kokkonen , N , Salo , S , Vayrynen , J P , Hurskainen , T , Manninen , A , Riihila , P , Heljasvaara , R , Franzke , C-W , Kahari , V-M , Salo , T , Makinen , M J & Tasanen , K 2017 , ' Significant Role of Collagen XVII And Integrin beta 4 in Migration and Invasion of The Less Aggressive Squamous Cell Carcinoma Cells ' , Scientific Reports , vol. 7 , 45057 . https://doi.org/10.1038/srep45057

Title: Significant Role of Collagen XVII And Integrin beta 4 in Migration and Invasion of The Less Aggressive Squamous Cell Carcinoma Cells
Author: Moilanen, Jyri M.; Loeffek, Stefanie; Kokkonen, Nina; Salo, Sirpa; Vayrynen, Juha P.; Hurskainen, Tiina; Manninen, Aki; Riihila, Pilvi; Heljasvaara, Ritva; Franzke, Claus-Werner; Kahari, Veli-Matti; Salo, Tuula; Makinen, Markus J.; Tasanen, Kaisa
Contributor organization: HUSLAB
Department of Oral and Maxillofacial Diseases
Clinicum
University of Helsinki
Medicum
Department of Pathology
Date: 2017-03-22
Language: eng
Number of pages: 11
Belongs to series: Scientific Reports
ISSN: 2045-2322
DOI: https://doi.org/10.1038/srep45057
URI: http://hdl.handle.net/10138/188605
Abstract: Collagen XVII and integrin alpha 6 beta 4 have well-established roles as epithelial adhesion molecules. Their binding partner laminin 332 as well as integrin alpha 6 beta 4 are largely recognized to promote invasion and metastasis in various cancers, and collagen XVII is essential for the survival of colon and lung cancer stem cells. We have studied the expression of laminin.2, collagen XVII and integrin beta 4 in tissue microarray samples of squamous cell carcinoma (SCC) and its precursors, actinic keratosis and Bowen's disease. The expression of laminin.2 was highest in SCC samples, whereas the expression of collagen XVII and integrin beta 4 varied greatly in SCC and its precursors. Collagen XVII and integrin beta 4 were also expressed in SCC cell lines. Virus-mediated RNAi knockdown of collagen XVII and integrin beta 4 reduced the migration of less aggressive SCC-25 cells in horizontal scratch wound healing assay. Additionally, in a 3D organotypic myoma invasion assay the loss of collagen XVII or integrin beta 4 suppressed equally the migration and invasion of SCC-25 cells whereas there was no effect on the most aggressive HSC-3 cells. Variable expression patterns and results in migration and invasion assays suggest that collagen XVII and integrin beta 4 contribute to SCC tumorigenesis.
Subject: ALPHA-6-BETA-4 INTEGRIN
TUMOR MICROENVIRONMENT
COLORECTAL-CANCER
RNA INTERFERENCE
EXPRESSION
SKIN
KERATINOCYTES
METASTASIS
GROWTH
PROGRESSION
3122 Cancers
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion


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