Maes , A , Gracia , C , Innocenti , N , Zhang , K , Aurell , E & Hajnsdorf , E 2017 , ' Landscape of RNA polyadenylation in E-coli ' , Nucleic Acids Research , vol. 45 , no. 5 , pp. 2746-2756 . https://doi.org/10.1093/nar/gkw894
Title: | Landscape of RNA polyadenylation in E-coli |
Author: | Maes, Alexandre; Gracia, Celine; Innocenti, Nicolas; Zhang, Kaiyang; Aurell, Erik; Hajnsdorf, Eliane |
Contributor organization: | Research Programs Unit Sampsa Hautaniemi / Principal Investigator University of Helsinki Genome-Scale Biology (GSB) Research Program |
Date: | 2017-03-17 |
Language: | eng |
Number of pages: | 11 |
Belongs to series: | Nucleic Acids Research |
ISSN: | 0305-1048 |
DOI: | https://doi.org/10.1093/nar/gkw894 |
URI: | http://hdl.handle.net/10138/188607 |
Abstract: | Polyadenylation is thought to be involved in the degradation and quality control of bacterial RNAs but relatively few examples have been investigated. We used a combination of 5 '-tagRACE and RNA-seq to analyze the total RNA content from a wild-type strain and from a poly(A) polymerase deleted Mutant. A total of 178 transcripts were either up- or down-regulated in the mutant when compared to the wild-type strain. Poly(A) polymerase up-regulates the expression of all genes related to the FliA regulon and several previously unknown transcripts, including numerous transporters. Notable down-regulation of genes in the expression of antigen 43 and components of the type 1 fimbriae was detected. The major consequence of the absence of poly(A) polymerase was the accumulation of numerous sRNAs, antisense transcripts, REP sequences and RNA fragments resulting from the processing of entire transcripts. A new algorithm to analyze the position and composition of post-transcriptional modifications based on the sequence of unencoded 3 '-ends, was developed to identify polyadenylated molecules. Overall our results shed new light on the broad spectrum of action of polyadenylation on gene expression and demonstrate the importance of poly(A) dependent degradation to remove structured RNA fragments. |
Subject: |
RPSO MESSENGER-RNA
POLY(A) POLYMERASE I POLYNUCLEOTIDE PHOSPHORYLASE POLY(A)-DEPENDENT DEGRADATION SEQUENCING REVEALS GENE-EXPRESSION DECAY IDENTIFICATION BACTERIA MOTILITY 3111 Biomedicine |
Peer reviewed: | Yes |
Rights: | cc_by_nc |
Usage restriction: | openAccess |
Self-archived version: | publishedVersion |
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