Landscape of RNA polyadenylation in E-coli

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Pysyväisosoite

http://hdl.handle.net/10138/188607

Lähdeviite

Maes , A , Gracia , C , Innocenti , N , Zhang , K , Aurell , E & Hajnsdorf , E 2017 , ' Landscape of RNA polyadenylation in E-coli ' , Nucleic Acids Research , vol. 45 , no. 5 , pp. 2746-2756 . https://doi.org/10.1093/nar/gkw894

Julkaisun nimi: Landscape of RNA polyadenylation in E-coli
Tekijä: Maes, Alexandre; Gracia, Celine; Innocenti, Nicolas; Zhang, Kaiyang; Aurell, Erik; Hajnsdorf, Eliane
Tekijän organisaatio: Research Programs Unit
Sampsa Hautaniemi / Principal Investigator
University of Helsinki
Genome-Scale Biology (GSB) Research Program
Päiväys: 2017-03-17
Kieli: eng
Sivumäärä: 11
Kuuluu julkaisusarjaan: Nucleic Acids Research
ISSN: 0305-1048
DOI-tunniste: https://doi.org/10.1093/nar/gkw894
URI: http://hdl.handle.net/10138/188607
Tiivistelmä: Polyadenylation is thought to be involved in the degradation and quality control of bacterial RNAs but relatively few examples have been investigated. We used a combination of 5 '-tagRACE and RNA-seq to analyze the total RNA content from a wild-type strain and from a poly(A) polymerase deleted Mutant. A total of 178 transcripts were either up- or down-regulated in the mutant when compared to the wild-type strain. Poly(A) polymerase up-regulates the expression of all genes related to the FliA regulon and several previously unknown transcripts, including numerous transporters. Notable down-regulation of genes in the expression of antigen 43 and components of the type 1 fimbriae was detected. The major consequence of the absence of poly(A) polymerase was the accumulation of numerous sRNAs, antisense transcripts, REP sequences and RNA fragments resulting from the processing of entire transcripts. A new algorithm to analyze the position and composition of post-transcriptional modifications based on the sequence of unencoded 3 '-ends, was developed to identify polyadenylated molecules. Overall our results shed new light on the broad spectrum of action of polyadenylation on gene expression and demonstrate the importance of poly(A) dependent degradation to remove structured RNA fragments.
Avainsanat: RPSO MESSENGER-RNA
POLY(A) POLYMERASE I
POLYNUCLEOTIDE PHOSPHORYLASE
POLY(A)-DEPENDENT DEGRADATION
SEQUENCING REVEALS
GENE-EXPRESSION
DECAY
IDENTIFICATION
BACTERIA
MOTILITY
3111 Biomedicine
Vertaisarvioitu: Kyllä
Tekijänoikeustiedot: cc_by_nc
Pääsyrajoitteet: openAccess
Rinnakkaistallennettu versio: publishedVersion


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