Landscape of RNA polyadenylation in E-coli

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dc.contributor.author Maes, Alexandre
dc.contributor.author Gracia, Celine
dc.contributor.author Innocenti, Nicolas
dc.contributor.author Zhang, Kaiyang
dc.contributor.author Aurell, Erik
dc.contributor.author Hajnsdorf, Eliane
dc.date.accessioned 2017-06-05T11:16:02Z
dc.date.available 2017-06-05T11:16:02Z
dc.date.issued 2017-03-17
dc.identifier.citation Maes , A , Gracia , C , Innocenti , N , Zhang , K , Aurell , E & Hajnsdorf , E 2017 , ' Landscape of RNA polyadenylation in E-coli ' , Nucleic Acids Research , vol. 45 , no. 5 , pp. 2746-2756 . https://doi.org/10.1093/nar/gkw894
dc.identifier.other PURE: 85156449
dc.identifier.other PURE UUID: 70aa887d-b0f3-4003-be09-b41686be8b98
dc.identifier.other WOS: 000397286600048
dc.identifier.other Scopus: 85018357344
dc.identifier.uri http://hdl.handle.net/10138/188607
dc.description.abstract Polyadenylation is thought to be involved in the degradation and quality control of bacterial RNAs but relatively few examples have been investigated. We used a combination of 5 '-tagRACE and RNA-seq to analyze the total RNA content from a wild-type strain and from a poly(A) polymerase deleted Mutant. A total of 178 transcripts were either up- or down-regulated in the mutant when compared to the wild-type strain. Poly(A) polymerase up-regulates the expression of all genes related to the FliA regulon and several previously unknown transcripts, including numerous transporters. Notable down-regulation of genes in the expression of antigen 43 and components of the type 1 fimbriae was detected. The major consequence of the absence of poly(A) polymerase was the accumulation of numerous sRNAs, antisense transcripts, REP sequences and RNA fragments resulting from the processing of entire transcripts. A new algorithm to analyze the position and composition of post-transcriptional modifications based on the sequence of unencoded 3 '-ends, was developed to identify polyadenylated molecules. Overall our results shed new light on the broad spectrum of action of polyadenylation on gene expression and demonstrate the importance of poly(A) dependent degradation to remove structured RNA fragments. en
dc.format.extent 11
dc.language.iso eng
dc.relation.ispartof Nucleic Acids Research
dc.rights cc_by_nc
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject RPSO MESSENGER-RNA
dc.subject POLY(A) POLYMERASE I
dc.subject POLYNUCLEOTIDE PHOSPHORYLASE
dc.subject POLY(A)-DEPENDENT DEGRADATION
dc.subject SEQUENCING REVEALS
dc.subject GENE-EXPRESSION
dc.subject DECAY
dc.subject IDENTIFICATION
dc.subject BACTERIA
dc.subject MOTILITY
dc.subject 3111 Biomedicine
dc.title Landscape of RNA polyadenylation in E-coli en
dc.type Article
dc.contributor.organization Research Programs Unit
dc.contributor.organization Sampsa Hautaniemi / Principal Investigator
dc.contributor.organization University of Helsinki
dc.contributor.organization Genome-Scale Biology (GSB) Research Program
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.1093/nar/gkw894
dc.relation.issn 0305-1048
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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