Complement Component C3 and Complement Factor B Promote Growth of Cutaneous Squamous Cell Carcinoma

Show simple item record Riihila, Pilvi Nissinen, Liisa Farshchian, Mehdi Kallajoki, Markku Kivisaari, Atte Meri, Seppo Grenman, Reidar Peltonen, Sirkku Peltonen, Juha Pihlajaniemi, Taina Heljasvaara, Ritva Kahari, Veli-Matti 2017-06-07T06:15:01Z 2017-06-07T06:15:01Z 2017-05
dc.identifier.citation Riihila , P , Nissinen , L , Farshchian , M , Kallajoki , M , Kivisaari , A , Meri , S , Grenman , R , Peltonen , S , Peltonen , J , Pihlajaniemi , T , Heljasvaara , R & Kahari , V-M 2017 , ' Complement Component C3 and Complement Factor B Promote Growth of Cutaneous Squamous Cell Carcinoma ' , The American Journal of Pathology , vol. 187 , no. 5 , pp. 1186-1197 .
dc.identifier.other PURE: 85254163
dc.identifier.other PURE UUID: e2e860ee-c425-48e1-9d73-7e71c0d3a53a
dc.identifier.other WOS: 000400229600020
dc.identifier.other Scopus: 85019036701
dc.description.abstract Cutaneous squamous cell carcinoma (cSCC) is one of the most common metastatic skin cancers with increasing incidence. We examined the roles of complement component C3 and complement factor B (CFB) in the growth of cSCC. Analysis of cSCC cell lines (n = 8) and normal human epidermal kerati-nocytes (n = 11) with real-time quantitative PCR and Western blotting revealed up-regulation of C3 and CFB expression in cSCC cells. Immunohistochemical staining revealed stronger tumor cell specific Labeling for C3 and CFB in invasive cSCCs (n = 71) and recessive dystrophic epidermolysis bullosa-associated cSCCs (n = 11) than in cSCC in situ (n = 69), actinic keratoses (n = 63), and normal skin (n = 5). Significant up-regulation of C3 and CFB mRNA expression was noted in chemically induced mouse cSCCs, compared to benign papillomas. Knockdown of C3 and CFB expression inhibited migration and proliferation of cSCC cells and resulted in potent inhibition of extracellular signal regulated kinase 1/2 activation. Knockdown of C3 and CFB markedly inhibited growth of human cSCC xenograft tumors in vivo. These results provide evidence for the rotes of C3 and CFB in the development of cSCC and identify them as biomarkers and potential therapeutic targets in this metastatic skin cancer. en
dc.format.extent 12
dc.language.iso eng
dc.relation.ispartof The American Journal of Pathology
dc.rights cc_by_nc_nd
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject LUNG-CANCER
dc.subject FACTOR-I
dc.subject FACTOR-H
dc.subject EXPRESSION
dc.subject PROGRESSION
dc.subject SKIN
dc.subject ACTIVATION
dc.subject 3111 Biomedicine
dc.title Complement Component C3 and Complement Factor B Promote Growth of Cutaneous Squamous Cell Carcinoma en
dc.type Article
dc.contributor.organization Medicum
dc.contributor.organization Seppo Meri / Principal Investigator
dc.contributor.organization Department of Bacteriology and Immunology
dc.description.reviewstatus Peer reviewed
dc.relation.issn 0002-9440
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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