Attenuated Semliki Forest virus for cancer treatment in dogs : safety assessment in two laboratory Beagles

Show full item record



Permalink

http://hdl.handle.net/10138/191760

Citation

Autio , K P M , Ruotsalainen , J J , Anttila , M O , Niittykoski , M , Waris , M , Hemminki , A , Vähä-Koskela , M J V & Hinkkanen , A E 2015 , ' Attenuated Semliki Forest virus for cancer treatment in dogs : safety assessment in two laboratory Beagles ' , BMC Veterinary Research , vol. 11 , 170 . https://doi.org/10.1186/s12917-015-0498-2

Title: Attenuated Semliki Forest virus for cancer treatment in dogs : safety assessment in two laboratory Beagles
Author: Autio, Karoliina P. M.; Ruotsalainen, Janne J.; Anttila, Marjukka O.; Niittykoski, Minna; Waris, Matti; Hemminki, Akseli; Vähä-Koskela, Markus J. V.; Hinkkanen, Ari E.
Contributor: University of Helsinki, Departments of Faculty of Veterinary Medicine
University of Helsinki, Medicum
University of Helsinki, Institute of Biotechnology
Date: 2015-07-28
Language: eng
Number of pages: 9
Belongs to series: BMC Veterinary Research
ISSN: 1746-6148
URI: http://hdl.handle.net/10138/191760
Abstract: Background: Dogs suffer from spontaneous tumors which may be amenable to therapies developed for human cancer patients, and dogs may serve as large-animal cancer models. A non-pathogenic Semliki Forest virus vector VA7-EGFP previously showed promise in targeting human tumor xenografts in mice, but the oncolytic capacity of the virus in canine cancer cells and the safety of the virus in higher mammals such as dogs, are not known. We therefore assessed the oncolytic potency of VA7-EGFP against canine cancer cells by infectivity and viability assays in two dog solid tumor cell lines. Furthermore we performed a 3-week safety study in two adult Beagles which received a single intravenous injection of similar to 2 x 10(5) plaque forming units of parental A7(74) strain. Results: VA7-EGFP was able to replicate in and kill both canine cancer cell lines tested. No adverse events were observed in either of the two virus-injected adult Beagles and no infective virus could be recovered from any of the biological samples collected over the course of the study. Neutralizing antibodies to Semliki Forest virus became detectable in the dogs at 5 days post infection and remained elevated until study termination. Conclusions: Based on these results, testing of the oncolytic potential of attenuated Semliki Forest virus in canine cancer patients appears feasible.
Subject: Oncolytic virus
Semliki Forest virus
Canine
Cancer
ONCOLYTIC ADENOVIRUS
VACCINIA VIRUS
A7(74) STRAIN
GENE-THERAPY
NUDE-MICE
DEMYELINATION
INFECTION
GLIOMA
REPLICATION
VIROTHERAPY
413 Veterinary science
3111 Biomedicine
Rights:


Files in this item

Total number of downloads: Loading...

Files Size Format View
Attenuated_Semliki.pdf 932.2Kb PDF View/Open

This item appears in the following Collection(s)

Show full item record