Genotypic and phenotypic diversity of Lactobacillus rhamnosus clinical isolates, their comparison with strain GG and their recognition by complement system

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Nissila , E , Douillard , F P , Ritari , J , Paulin , L , Jarvinen , H M , Rasinkangas , P , Haapasalo , K , Meri , S , Jarva , H & de Vos , W M 2017 , ' Genotypic and phenotypic diversity of Lactobacillus rhamnosus clinical isolates, their comparison with strain GG and their recognition by complement system ' , PLoS One , vol. 12 , no. 5 , 0176739 . https://doi.org/10.1371/journal.pone.0176739

Title: Genotypic and phenotypic diversity of Lactobacillus rhamnosus clinical isolates, their comparison with strain GG and their recognition by complement system
Author: Nissila, Eija; Douillard, Francois P.; Ritari, Jarmo; Paulin, Lars; Jarvinen, Hanna M.; Rasinkangas, Pia; Haapasalo, Karita; Meri, Seppo; Jarva, Hanna; de Vos, Willem M.
Contributor organization: Research Programs Unit
Medicum
Immunobiology Research Program
Department of Bacteriology and Immunology
Immunomics
Departments of Faculty of Veterinary Medicine
Veterinary Biosciences
Veterinary Microbiology and Epidemiology
Institute of Biotechnology
Biosciences
HUSLAB
Seppo Meri / Principal Investigator
Clinicum
Willem Meindert Vos de / Principal Investigator
de Vos & Salonen group
Date: 2017-05-11
Language: eng
Number of pages: 18
Belongs to series: PLoS One
ISSN: 1932-6203
DOI: https://doi.org/10.1371/journal.pone.0176739
URI: http://hdl.handle.net/10138/193013
Abstract: Lactobacillus rhamnosus strains are ubiquitous in fermented foods, and in the human body where they are commensals naturally present in the normal microbiota composition of gut, vagina and skin. However, in some cases, Lactobacillus spp. have been implicated in bacteremia. The aim of the study was to examine the genomic and immunological properties of 16 clinical blood isolates of L. rhamnosus and to compare them to the well- studied L. rhamnosus probiotic strain GG. Blood cultures from bacteremic patients were collected at the Helsinki University Hospital laboratory in 2005-2011 and L. rhamnosus strains were isolated and characterized by genomic sequencing. The capacity of the L. rhamnosus strains to activate serum complement was studied using immunological assays for complement factor C3a and the terminal pathway complement complex (TCC). Binding of complement regulators factor H and C4bp was also determined using radioligand assays. Furthermore, the isolated strains were evaluated for their ability to aggregate platelets and to form biofilms in vitro. Genomic comparison between the clinical L. rhamnosus strains showed them to be clearly different from L. rhamnosus GG and to cluster in two distinct lineages. All L. rhamnosus strains activated complement in serum and none of them bound complement regulators. Four out of 16 clinical blood isolates induced platelet aggregation and/or formed more biofilms than L. rhamnosus GG, which did not display platelet aggregation activity nor showed strong biofilm formation. These findings suggest that clinical L. rhamnosus isolates show considerable heterogeneity but are clearly different from L. rhamnosus GG at the genomic level. All L. rhamnosus strains are still normally recognized by the human complement system.
Subject: STREPTOCOCCUS-PYOGENES INFECTIONS
LACTIC-ACID BACTERIA
FACTOR-H
BIOFILM FORMATION
BINDING-PROTEIN
IN-VITRO
PLATELET ACTIVATION
PROBIOTIC USE
AGGREGATION
ADHESION
3111 Biomedicine
1183 Plant biology, microbiology, virology
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion


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