Pathogenic seedborne viruses are rare but Phaseolus vulgaris endornaviruses are common in bean varieties grown in Nicaragua and Tanzania

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Nordenstedt , N , Marcenaro , D , Chilagane , D , Mwaipopo , B , Rajamaki , M-L , Nchimbi-Msolla , S , Njau , P J R , Mbanzibwa , D R & Valkonen , J P T 2017 , ' Pathogenic seedborne viruses are rare but Phaseolus vulgaris endornaviruses are common in bean varieties grown in Nicaragua and Tanzania ' , PLoS One , vol. 12 , no. 5 , 0178242 . https://doi.org/10.1371/journal.pone.0178242

Title: Pathogenic seedborne viruses are rare but Phaseolus vulgaris endornaviruses are common in bean varieties grown in Nicaragua and Tanzania
Author: Nordenstedt, Noora; Marcenaro, Delfia; Chilagane, Daudi; Mwaipopo, Beatrice; Rajamaki, Minna-Liisa; Nchimbi-Msolla, Susan; Njau, Paul J. R.; Mbanzibwa, Deusdedith R.; Valkonen, Jari P. T.
Contributor organization: Department of Agricultural Sciences
Plant Pathology and Virology
Viikki Plant Science Centre (ViPS)
Plant Production Sciences
Date: 2017-05-25
Language: eng
Number of pages: 18
Belongs to series: PLoS One
ISSN: 1932-6203
DOI: https://doi.org/10.1371/journal.pone.0178242
URI: http://hdl.handle.net/10138/193779
Abstract: Common bean (Phaseolus vulgaris) is an annual grain legume that was domesticated in Mesoamerica (Central America) and the Andes. It is currently grown widely also on other continents including Africa. We surveyed seedborne viruses in new common bean varieties introduced to Nicaragua (Central America) and in landraces and improved varieties grown in Tanzania (eastern Africa). Bean seeds, harvested from Nicaragua and Tanzania, were grown in insect-controlled greenhouse or screenhouse, respectively, to obtain leaf material for virus testing. Equal amounts of total RNA from different samples were pooled (30-36 samples per pool), and small RNAs were deep-sequenced (Illumina). Assembly of the reads (21-24 nt) to contiguous sequences and searches for homologous viral sequences in data-bases revealed Phaseolus vulgaris endornavirus 1 (PvEV-1) and PvEV-2 in the bean varieties in Nicaragua and Tanzania. These viruses are not known to cause symptoms in common bean and are considered non-pathogenic. The small-RNA reads from each pool of samples were mapped to the previously characterized complete PvEV-1 and PvEV-2 sequences (genome lengths ca. 14 kb and 15 kb, respectively). Coverage of the viral genomes was 87.9-99.9%, depending on the pool. Coverage per nucleotide ranged from 5 to 471, confirming virus identification. PvEV-1 and PvEV-2 are known to occur in Phaseolus spp. in Central America, but there is little previous information about their occurrence in Nicaragua, and no information about occurrence in Africa. Aside from Cowpea mild mosaic virus detected in bean plants grown from been seeds harvested from one region in Tanzania, no other pathogenic seedborne viruses were detected. The low incidence of infections caused by pathogenic viruses transmitted via bean seeds may be attributable to new, virus-resistant CB varieties released by breeding programs in Nicaragua and Tanzania.
Subject: MOSAIC-NECROSIS-VIRUS
CYTOPLASMIC MALE-STERILITY
POLYMERASE-CHAIN-REACTION
BELL PEPPER ENDORNAVIRUS
SMALL INTERFERING RNAS
NUCLEOTIDE-SEQUENCE
DEGENERATE PRIMERS
GERMPLASM LINES
GENOME SEQUENCE
VICIA-FABA
1183 Plant biology, microbiology, virology
414 Agricultural biotechnology
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion


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