Gustafsson , M O , Mohammad , D K , Ylosmaki , E , Choi , H , Shrestha , S , Wang , Q , Nore , B F , Saksela , K & Smith , C I E 2017 , ' ANKRD54 preferentially selects Bruton's Tyrosine Kinase (BTK) from a Human Src-Homology 3 (SH3) domain library ' , PLoS One , vol. 12 , no. 4 , 0174909 . https://doi.org/10.1371/journal.pone.0174909
Title: | ANKRD54 preferentially selects Bruton's Tyrosine Kinase (BTK) from a Human Src-Homology 3 (SH3) domain library |
Author: | Gustafsson, Manuela O.; Mohammad, Dara K.; Ylosmaki, Erkko; Choi, Hyunseok; Shrestha, Subhash; Wang, Qing; Nore, Beston F.; Saksela, Kalle; Smith, C. I. Edvard |
Contributor organization: | Medicum Department of Virology University of Helsinki Clinicum Kalle Saksela / Principal Investigator ImmunoViroTherapy Lab HUSLAB |
Date: | 2017-04-03 |
Language: | eng |
Number of pages: | 13 |
Belongs to series: | PLoS One |
ISSN: | 1932-6203 |
DOI: | https://doi.org/10.1371/journal.pone.0174909 |
URI: | http://hdl.handle.net/10138/195632 |
Abstract: | Bruton's Tyrosine Kinase (BTK) is a cytoplasmic protein tyrosine kinase with a fundamental role in B-lymphocyte development and activation. The nucleocytoplasmic shuttling of BTK is specifically modulated by the Ankyrin Repeat Domain 54 (ANKRD54) protein and the interaction is known to be exclusively SH3-dependent. To identify the spectrum of the ANKRD54 SH3-interactome, we applied phage-display screening of a library containing all the 296 human SH3 domains. The BTK-SH3 domain was the prime interactor. Quantitative western blotting analysis demonstrated the accuracy of the screening procedure. Revealing the spectrum and specificity of ANKRD54-interactome is a critical step toward functional analysis in cells and tissues. |
Subject: |
ANKYRIN REPEAT
TEC FAMILY LIGAND-BINDING PROTEIN PHOSPHORYLATION IDENTIFICATION EXPRESSION MOTIF AGAMMAGLOBULINEMIA LYMPHOCYTES 3111 Biomedicine |
Peer reviewed: | Yes |
Rights: | cc_by |
Usage restriction: | openAccess |
Self-archived version: | publishedVersion |
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