ANKRD54 preferentially selects Bruton's Tyrosine Kinase (BTK) from a Human Src-Homology 3 (SH3) domain library

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Gustafsson , M O , Mohammad , D K , Ylosmaki , E , Choi , H , Shrestha , S , Wang , Q , Nore , B F , Saksela , K & Smith , C I E 2017 , ' ANKRD54 preferentially selects Bruton's Tyrosine Kinase (BTK) from a Human Src-Homology 3 (SH3) domain library ' , PLoS One , vol. 12 , no. 4 , 0174909 . https://doi.org/10.1371/journal.pone.0174909

Title: ANKRD54 preferentially selects Bruton's Tyrosine Kinase (BTK) from a Human Src-Homology 3 (SH3) domain library
Author: Gustafsson, Manuela O.; Mohammad, Dara K.; Ylosmaki, Erkko; Choi, Hyunseok; Shrestha, Subhash; Wang, Qing; Nore, Beston F.; Saksela, Kalle; Smith, C. I. Edvard
Contributor: University of Helsinki, Medicum
University of Helsinki, Medicum
University of Helsinki, Medicum
Date: 2017-04-03
Language: eng
Number of pages: 13
Belongs to series: PLoS One
ISSN: 1932-6203
URI: http://hdl.handle.net/10138/195632
Abstract: Bruton's Tyrosine Kinase (BTK) is a cytoplasmic protein tyrosine kinase with a fundamental role in B-lymphocyte development and activation. The nucleocytoplasmic shuttling of BTK is specifically modulated by the Ankyrin Repeat Domain 54 (ANKRD54) protein and the interaction is known to be exclusively SH3-dependent. To identify the spectrum of the ANKRD54 SH3-interactome, we applied phage-display screening of a library containing all the 296 human SH3 domains. The BTK-SH3 domain was the prime interactor. Quantitative western blotting analysis demonstrated the accuracy of the screening procedure. Revealing the spectrum and specificity of ANKRD54-interactome is a critical step toward functional analysis in cells and tissues.
Subject: ANKYRIN REPEAT
TEC FAMILY
LIGAND-BINDING
PROTEIN
PHOSPHORYLATION
IDENTIFICATION
EXPRESSION
MOTIF
AGAMMAGLOBULINEMIA
LYMPHOCYTES
3111 Biomedicine
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