Multiplex detection in tonsillar tissue of all known human polyomaviruses

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Sadeghi , M , Wang , Y , Ramqvist , T , Aaltonen , L-M , Pyöriä , L , Toppinen , M , Soderlund-Venermo , M & Hedman , K 2017 , ' Multiplex detection in tonsillar tissue of all known human polyomaviruses ' , BMC Infectious Diseases , vol. 17 , 409 . https://doi.org/10.1186/s12879-017-2479-5

Title: Multiplex detection in tonsillar tissue of all known human polyomaviruses
Author: Sadeghi, Mohammadreza; Wang, Yilin; Ramqvist, Torbjorn; Aaltonen, Leena-Maija; Pyöriä, Lari; Toppinen, Mari; Soderlund-Venermo, Maria; Hedman, Klaus
Contributor: University of Helsinki, Department of Virology
University of Helsinki, Medicum
University of Helsinki, Korva-, nenä- ja kurkkutautien klinikka
University of Helsinki, Medicum
University of Helsinki, University of Helsinki
University of Helsinki, Medicum
University of Helsinki, HUSLAB
Date: 2017-06-08
Language: eng
Number of pages: 8
Belongs to series: BMC Infectious Diseases
ISSN: 1471-2334
URI: http://hdl.handle.net/10138/198879
Abstract: Background: In the past few years, eleven new human viruses have joined the two previously known members JCPyV and BKPyV of the Polyomaviridae family, by virtue of molecular methods. Serology data suggest that infections with human polyomaviruses (HPyVs) occur since childhood and the viruses are widespread in the general population. However, the viral persistence sites and transmission routes are by and large unknown. Our previous studies demonstrated that the four new HPyVs KIPyV, WUPyV, MCPyV and TSPyV were present in the tonsils, and suggested lymphoid tissue as a persistent site of these emerging human viruses. We developed a Luminex-based multiplex assay for simultaneous detection of all 13 HPyVs known, and explored their occurrence in tonsillar tissues of children and adults mostly with tonsillitis or tonsillar hypertrophy. Methods: We set up and validated a new Luminex-based multiplex assay by using primer pairs and probes targeting the respective HPyV viral protein 1 (VP1) genes. With this assay we tested 78 tonsillar tissues for DNAs of 13 HPyVs. Results: The multiplex assay allowed for simultaneous detection of 13 HPyVs with high analytical sensitivity and specificity, with detection limits of 10(0)-10(2) copies per microliter, and identified correctly all 13 target sequences with no cross reactions. HPyV DNA altogether was found in 14 (17.9%) of 78 tonsils. The most prevalent HPyVs were HPyV6 (7.7%), TSPyV (3.8%) and WUPyV (3.8%). Mixed infection of two HPyVs occurred in one sample. Conclusions: The Luminex-based HPyV multiplex assay appears highly suitable for clinical diagnostic purposes and large-scale epidemiological studies. Additional evidence was acquired that the lymphoid system plays a role in HPyV infection and persistence. Thereby, shedding from this site during reactivation might take part in transmission of the newly found HPyVs.
Subject: HPyV
PCR
Luminex
Tonsil
SPINULOSA-ASSOCIATED POLYOMAVIRUS
MERKEL CELL POLYOMAVIRUS
PROGRESSIVE MULTIFOCAL LEUKOENCEPHALOPATHY
CLINICAL-IMPLICATIONS
JC VIRUS
CROSS-REACTIVITY
VIRAL LATENCY
KI
INFECTION
DNA
3121 General medicine, internal medicine and other clinical medicine
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