Locally Triggered Release of the Chemokine CCL21 Promotes Dendritic Cell Transmigration across Lymphatic Endothelia

Show full item record



Permalink

http://hdl.handle.net/10138/198912

Citation

Vaahtomeri , K , Brown , M , Hauschild , R , De Vries , I , Leithner , A F , Mehling , M , Kaufmann , W A & Sixt , M 2017 , ' Locally Triggered Release of the Chemokine CCL21 Promotes Dendritic Cell Transmigration across Lymphatic Endothelia ' , Cell Reports , vol. 19 , no. 5 , pp. 902-909 . https://doi.org/10.1016/j.celrep.2017.04.027

Title: Locally Triggered Release of the Chemokine CCL21 Promotes Dendritic Cell Transmigration across Lymphatic Endothelia
Author: Vaahtomeri, Kari; Brown, Markus; Hauschild, Robert; De Vries, Ingrid; Leithner, Alexander Franz; Mehling, Matthias; Kaufmann, Walter Anton; Sixt, Michael
Contributor: University of Helsinki, Research Programs Unit
Date: 2017-05-02
Language: eng
Number of pages: 8
Belongs to series: Cell Reports
ISSN: 2211-1247
URI: http://hdl.handle.net/10138/198912
Abstract: Trafficking cells frequently transmigrate through epithelial and endothelial monolayers. How monolayers cooperate with the penetrating cells to support their transit is poorly understood. We studied dendritic cell (DC) entry into lymphatic capillaries as a model system for transendothelial migration. We find that the chemokine CCL21, which is the decisive guidance cue for intravasation, mainly localizes in the trans-Golgi network and intracellular vesicles of lymphatic endothelial cells. Upon DC transmigration, these Golgi deposits disperse and CCL21 becomes extracellularly enriched at the sites of endothelial cell-cell junctions. When we reconstitute the transmigration process in vitro, we find that secretion of CCL21-positive vesicles is triggered by a DC contact-induced calcium signal, and selective calcium chelation in lymphatic endothelium attenuates transmigration. Altogether, our data demonstrate a chemokine-mediated feedback between DCs and lymphatic endothelium, which facilitates transendothelial migration.
Subject: BETA-CELLS
TRANSENDOTHELIAL MIGRATION
INSULIN-SECRETION
VESICLE MOVEMENTS
CHROMAFFIN CELLS
MYCALOLIDE-B
INFLAMMATION
VESSELS
CCR7
MICROTUBULES
3111 Biomedicine
1182 Biochemistry, cell and molecular biology
Rights:


Files in this item

Total number of downloads: Loading...

Files Size Format View
1_s2.0_S2211124717305211_main.pdf 2.144Mb PDF View/Open

This item appears in the following Collection(s)

Show full item record