Pharmacological Preconditioning with Diazoxide in the Experimental Hypothermic Circulatory Arrest Model

Show full item record



Permalink

http://hdl.handle.net/10138/199525

Citation

Haapanen , H , Arvola , O , Herajarvi , J , Anttila , T , Tuominen , H , Puistola , U , Karihtala , P , Kiviluoma , K , Juvonen , T & Anttila , V 2017 , ' Pharmacological Preconditioning with Diazoxide in the Experimental Hypothermic Circulatory Arrest Model ' , Heart Surgery Forum , vol. 20 , no. 2 , pp. E69-E76 . https://doi.org/10.1532/hsf.1717

Title: Pharmacological Preconditioning with Diazoxide in the Experimental Hypothermic Circulatory Arrest Model
Author: Haapanen, Henri; Arvola, Oiva; Herajarvi, Johanna; Anttila, Tuomas; Tuominen, Hannu; Puistola, Ulla; Karihtala, Peeter; Kiviluoma, Kai; Juvonen, Tatu; Anttila, Vesa
Contributor: University of Helsinki, Clinicum
Date: 2017
Language: eng
Number of pages: 8
Belongs to series: Heart Surgery Forum
ISSN: 1098-3511
URI: http://hdl.handle.net/10138/199525
Abstract: Background: Hypothermic circulatory arrest includes a remarkable risk for neurological injury. Diazoxide, a mitochondrial adenosine triphosphate-dependent potassium ion (K+ATP) channel opener, is known to have cardioprotective effects. We assessed its efficacy in preventing ischemic injury in a clinically relevant animal model. Methods: Eighteen piglets were randomized into a diazoxide group (n = 9) and a control group (n = 9). Animals underwent 60 minutes of hypothermic circulatory arrest at 18 degrees C. Diazoxide (5 mg/kg + 10 mL NaOH + 40 mL NaCl) was infused during the cooling phase. Metabolic and hemodynamic data were collected throughout the experiment. After 24-hour follow-up, whole brain, heart, and kidney biopsy specimens were collected for analysis. Results: Cerebellar Cytochrome-C and caspase-3 activation was higher in the control group (P = .02 and P = .016, respectively). Antioxidant activity tended to be higher in the diazoxide group (P = .099). Throughout the experiment, the oxygen consumption ratio was higher in the control animals (P-g = .04), as were the lactate levels (P-g = .02). Cardiac function tended to be better in diazoxide-treated animals. Conclusion: Diazoxide might confer neuroprotective effect as implied by the immunohistochemical analysis of the brain. Additionally, the circulatory effects of diazoxide were beneficial, supporting its neuroprotective effect.
Subject: SENSITIVE K+ CHANNELS
HEART-MITOCHONDRIA
ATP CHANNELS
BRAIN
3121 General medicine, internal medicine and other clinical medicine
Rights:


Files in this item

Total number of downloads: Loading...

Files Size Format View
Pharmacological_Preconditioning_with_Diazoxide.pdf 781.1Kb PDF View/Open

This item appears in the following Collection(s)

Show full item record